Effects of Cholesterol on Progesterone Production by Goat Luteal Cell Subpopulations at Two Different Stages of the Luteal Phase

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dc.contributor.authorArikan, S.
dc.contributor.authorKalender, H.
dc.contributor.authorSimsek, O.
dc.date.accessioned2020-06-25T17:49:05Z
dc.date.available2020-06-25T17:49:05Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.description.abstractContents The aim of the present study was to evaluate the effects of cholesterol on progesterone production during long-term culturing of luteal cell subpopulations at early and late luteal stages of the goat corpora lutea. Corpora lutea were collected from Angora goats on days 5 and 15 of the oestrous cycle. Luteal cells were isolated by collagenase digestion. The cells were separated into two distinct subpopulations by Percoll density-gradient centrifugation. Both subpopulations of luteal cells staining positively for 3 beta-HSD activities (5 x 104 cell/well) were cultured with or without 22(R)-hydroxycholesterol (22R-HC) in serum-free culture medium for periods of up to 7 days. Cells were incubated with serum (10%) for the first 18 h of incubation followed by serum-free medium. Cell treatment (10 and 20 mu g/ml) was performed on days 1, 3 and 5. Treatment of cells with both concentrations of 22R-HC resulted in significant (p < 0.01) and dose-dependent stimulation (p > 0.05) on progesterone production in both fractions of cells throughout 7 days of incubation. Treatment of the cells with cholesterol resulted in 2.5- and 9.0-fold increases in progesterone accumulation on day 3 of incubation. Steroid production was maintained throughout the incubations when cells are incubated in serum-free media treated with cholesterol and ITS premix. Cells collected from higher density of percoll layers produced 2.82 and 2.32 times more progesterone, in comparison to the lover density percoll layer, on days 5 and 15 of the oestrous cycle in untreated cell groups, respectively. Progesterone accumulation was decreased as incubation time advanced in all groups of untreated cells. These results demonstrated that goat luteal cell subpopulations secrete substantial amounts of progesterone in response to cholesterol treatment at least for 7 days, and cholesterol is required as progesterone precursor for maintaining a high-level steroidogenesis during long-life culturing of both cell subpopulations.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-108O520]en_US
dc.description.sponsorshipFinancial support for present project was provided by the Scientific and Technological Research Council of Turkey (TUBITAK). Project No: TOVAG-108O520. The authors would like to thank Dr Mehmet Basalan for his assistance in statistical analysis.en_US
dc.identifier.citationBu makale açık erişimli değildir.en_US
dc.identifier.doi10.1111/j.1439-0531.2010.01596.x
dc.identifier.endpagee439en_US
dc.identifier.issn0936-6768
dc.identifier.issue6en_US
dc.identifier.pmid20345591
dc.identifier.scopus2-s2.0-78649530927
dc.identifier.scopusqualityQ2
dc.identifier.startpagee434en_US
dc.identifier.urihttps://doi.org/10.1111/j.1439-0531.2010.01596.x
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4631
dc.identifier.volume45en_US
dc.identifier.wosWOS:000284429900028
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-Blackwell Publishing, Incen_US
dc.relation.ispartofReproduction In Domestic Animals
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleEffects of Cholesterol on Progesterone Production by Goat Luteal Cell Subpopulations at Two Different Stages of the Luteal Phaseen_US
dc.typeArticle

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