Hydrolysis of sucrose by invertase immobilized onto novel magnetic polyvinylalcohol microspheres

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dc.contributor.authorAkgöl, S.
dc.contributor.authorKaçar, Y.
dc.contributor.authorDenizli, A.
dc.contributor.authorArıca, M.Y.
dc.date.accessioned2020-06-25T17:34:50Z
dc.date.available2020-06-25T17:34:50Z
dc.date.issued2001
dc.departmentKırıkkale Üniversitesi
dc.descriptionAkgol, Sinan/0000-0002-8528-1854; Kacar, Yasemin/0000-0002-8682-9228; AKGOL, Sinan/0000-0003-2836-7181
dc.description.abstractThe magnetic polyvinylalcohol (PVAL) microspheres were prepared by crosslinking glutaraldehyde. 1,1 ' -Carbonyldiimidazole (CDI), a carbonylating agent was used for the activation of hydroxyl groups of polyvinylalcohol, and invertase immobilized onto the magnetic PVAL microspheres by covalent bonding through the amino group. The retained activity of the immobilized invertase was 74%. Kinetic parameters were determined for immobilized invertase, as well as for the free enzyme. The K-m values for immobilized invertase (55 mM sucrose) were higher than that of the free enzyme (24 mM sucrose), whereas V-max values were smaller for the immobilized invertase. The optimum operational temperature was 5 degreesC higher for immobilized enzyme than that of the free enzyme. The operational inactivation rate constant (k(opi)) of the immobilized invertase at 35 degreesC with 200 mM sucrose was 5.83 x 10(-5) min(-1). Thermal and storage stabilities were found to increase with immobilization. (C) 2001 Elsevier Science Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0308-8146(01)00150-9
dc.identifier.endpage288en_US
dc.identifier.issn0308-8146
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0034884917
dc.identifier.scopusqualityQ1
dc.identifier.startpage281en_US
dc.identifier.urihttps://doi.org/10.1016/S0308-8146(01)00150-9
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2916
dc.identifier.volume74en_US
dc.identifier.wosWOS:000170507600004
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofFood Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpolyvinylalcoholen_US
dc.subjectmagnetiteen_US
dc.subjectmicrospheresen_US
dc.subjectcovalent bondingen_US
dc.subjectenzyme immobilizationen_US
dc.subjectinvertaseen_US
dc.titleHydrolysis of sucrose by invertase immobilized onto novel magnetic polyvinylalcohol microspheresen_US
dc.typeArticle

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