Poly(styrene-hydroxyethyl methacrylate) monodisperse microspheres as specific sorbent in dye affinity adsorption of albumin

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dc.contributor.authorUzun, L
dc.contributor.authorOdabasi, M
dc.contributor.authorArica, Y
dc.contributor.authorDenizli, A
dc.date.accessioned2020-06-25T17:40:06Z
dc.date.available2020-06-25T17:40:06Z
dc.date.issued2004
dc.departmentKırıkkale Üniversitesi
dc.descriptionUzun, Lokman/0000-0002-3971-7725
dc.description.abstractIn this study, human serum albumin (HSA) adsorption properties of reactive green HE-4BD-attached monodisperse poly(styrene-2-hydroxyethyl methacrylate) [poly(St-HEMA)] microspheres were investigated. Poly(St-HEMA) microspheres with an uniform size of 4.0 mum in diameter were produced by the dispersion copolymerization of St and HEMA in an ethanol-water medium. Reactive green HE-4BD was covalently attached onto the poly(St-HEMA) microspheres via a nucleophilic substitution reaction between the hydroxyl groups of HEMA and triazinyl chloro groups of dye with the equilibrium coupling capacity of 45.8 mumol dye (g polymer)(-1). The poly(St-HEMA) monodisperse microspheres were characterized by scanning electron microscopy. The effect of concentration of HSA, medium pH, and ionic strength on the adsorption efficiency of dye-attached microspheres were studied in a batch system. The nonspecific adsorption of HSA on the poly(St-HEMA) microspheres was 2.2 mg g(-1). Reactive green HE-4BD attachment significantly increased the HSA adsorption up to 221 mg g(-1). The langmuir adsorption model was found to be applicable in interpreting HSA adsorption by reactive green HE-4BD attached microspheres. Significant amount Of the adsorbed HSA (up to 92%) was eluted in 1 hr in the elution medium containing 1.0-M NaCl. To determine the effects of adsorption conditions on possible conformational changes of HSA structure, fluorescence spectrophotometry was employed. We resulted that poly(St-HEMA) dye-affinity microspheres can be applied for HSA adsorption without causing any significant conformational changes. Repeated adsorption/ elution processes showed that these dye-attached monodisperse microspheres are suitable for HSA adsorption.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1081/SS-120035934
dc.identifier.endpage2418en_US
dc.identifier.issn0149-6395
dc.identifier.issn1520-5754
dc.identifier.issue10en_US
dc.identifier.scopus2-s2.0-3843091674
dc.identifier.scopusqualityQ2
dc.identifier.startpage2401en_US
dc.identifier.urihttps://doi.org/10.1081/SS-120035934
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3276
dc.identifier.volume39en_US
dc.identifier.wosWOS:000223227800008
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofSeparation Science And Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titlePoly(styrene-hydroxyethyl methacrylate) monodisperse microspheres as specific sorbent in dye affinity adsorption of albuminen_US
dc.typeArticle

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