Expression of microRNA and microRNA processing machinery genes during early quail (Coturnix japonica) embryo development

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dc.contributor.authorKocamış, Hakan
dc.contributor.authorHossain, Md. M.
dc.contributor.authorÇınar, M. U.
dc.contributor.authorSalilew-Wondim, D.
dc.contributor.authorMohammadi-Sangcheshmeh, A.
dc.contributor.authorTesfaye, D.
dc.contributor.authorSchellander, K.
dc.date.accessioned2020-06-25T18:07:25Z
dc.date.available2020-06-25T18:07:25Z
dc.date.issued2013
dc.departmentKırıkkale Üniversitesi
dc.descriptionWondim, Dr. Dessie Salilew/0000-0002-8756-0865
dc.description.abstractMicroRNA (miRNA) are small regulatory RNA molecules that are implicated in regulating and controlling a wide range of physiological processes including cell division, differentiation, migration, apoptosis, morphogenesis, and organogenesis. The aim of this study was to determine the expression pattern of 32 miRNA and 18 miRNA processing machinery genes during somite formation in quail embryos. The embryos were collected at stages HH (Hamburger and Hamilton) 4, 6, and 9 of embryo development (19, 24, and 30 h of incubation, respectively). Total RNA including miRNA was isolated from 4 groups of embryos (each group consisting of 6 to 8 embryos) were collected at each of the 3 stages (19, 24, and 30 h). The expression pattern of candidate miRNA and miRNA processing machinery genes was performed using quantitative real-time PCR. The results demonstrated that 7 miRNA (let-7a, mir-122, mir-125b, mir-10b, P < 0.01; let-7b, mir-26a, and mir-126, P < 0.05) were differentially expressed during early quail embryo development. In addition, the expression profile of 18 miRNA processing machinery genes was not significantly increased at 30 h of incubation compared with both 19 and 24 h. Our results suggest that machinery genes for miRNA biogenetic pathways are functional, and hence, miRNA may be involved in the regulation of early quail development. These 7 differentially expressed miRNA are suggested to play critical roles in quail embryo somite formation.en_US
dc.identifier.citationKocamis, H., Hossain, M., Cinar, M. U., Salilew-Wondim, D., Mohammadi-Sangcheshmeh, A., Tesfaye, D., Hölker, M., & Schellander, K. (2013). Expression of microRNA and microRNA processing machinery genes during early quail (Coturnix japonica) embryo development. Poultry science, 92(3), 787–797. https://doi.org/10.3382/ps.2012-02691en_US
dc.identifier.doi10.3382/ps.2012-02691
dc.identifier.endpage797en_US
dc.identifier.issn0032-5791
dc.identifier.issn1525-3171
dc.identifier.issue3en_US
dc.identifier.pmid23436530
dc.identifier.scopus2-s2.0-84874415761
dc.identifier.scopusqualityQ1
dc.identifier.startpage787en_US
dc.identifier.urihttps://doi.org/10.3382/ps.2012-02691
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5570
dc.identifier.volume92en_US
dc.identifier.wosWOS:000319314800023
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofPoultry Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmicroRNAen_US
dc.subjectprocessing machinery geneen_US
dc.subjectquailen_US
dc.subjectembryoen_US
dc.subjectdevelopmenten_US
dc.titleExpression of microRNA and microRNA processing machinery genes during early quail (Coturnix japonica) embryo developmenten_US
dc.typeArticle

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