Molecular analysis of three local silkworm breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE methods
Yükleniyor...
Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Veteriner Fakultesi Dergisi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, PCR-based RAPD method was used to determine the genetic variation in three Turkish local silkworm breeds (Alaca, Bursa Beyazı and Hatay Sarısı) and SDS-PAGE method was used to analyse the sericin proteins of their cocoons. Three local breeds were analysed by randomly chosen 40 primers and 68 total RAPD bands observed with 7 of them. Percentages of polymorphic loci were determined higher in Hatay Sarısı (55.88), lower in Bursa Beyazı and Alaca (44.12). Nei's genetic distance was determined 0.0637 between Bursa Beyazı and Alaca, 0.1012 between Bursa Beyazı and Hatay Sarısı, 0.0793 between Alaca and Hatay Sarısı. In SDS-PAGE sericin proteins of three breeds was yielded a single band of 200 kDa.
Açıklama
Anahtar Kelimeler
Bombyx mori, Genetic polymorphism, RAPD-PCR, SDS-PAGE
Kaynak
Kafkas Universitesi Veteriner Fakultesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
16
Sayı
SUPPL. B
Künye
Akkir, D. & Budak Yıldıran, F Azize & Çakir, S.. (2010). Molecular analysis of three local silkworm breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE methods. Kafkas Universitesi Veteriner Fakultesi Dergisi. 16: S265-S269.
