Molecular analysis of three local silkworm breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE methods

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dc.contributor.authorEroğlu Akkir D.
dc.contributor.authorBudak Yildiran F.A.
dc.contributor.authorÇakir S.
dc.date.accessioned2020-06-25T15:14:36Z
dc.date.available2020-06-25T15:14:36Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, PCR-based RAPD method was used to determine the genetic variation in three Turkish local silkworm breeds (Alaca, Bursa Beyazı and Hatay Sarısı) and SDS-PAGE method was used to analyse the sericin proteins of their cocoons. Three local breeds were analysed by randomly chosen 40 primers and 68 total RAPD bands observed with 7 of them. Percentages of polymorphic loci were determined higher in Hatay Sarısı (55.88), lower in Bursa Beyazı and Alaca (44.12). Nei's genetic distance was determined 0.0637 between Bursa Beyazı and Alaca, 0.1012 between Bursa Beyazı and Hatay Sarısı, 0.0793 between Alaca and Hatay Sarısı. In SDS-PAGE sericin proteins of three breeds was yielded a single band of 200 kDa.en_US
dc.identifier.citationAkkir, D. & Budak Yıldıran, F Azize & Çakir, S.. (2010). Molecular analysis of three local silkworm breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE methods. Kafkas Universitesi Veteriner Fakultesi Dergisi. 16: S265-S269.en_US
dc.identifier.endpageS269en_US
dc.identifier.issn13006045
dc.identifier.issueSUPPL. Ben_US
dc.identifier.scopus2-s2.0-77956900527
dc.identifier.scopusqualityQ3
dc.identifier.startpageS265en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2152
dc.identifier.volume16en_US
dc.indekslendigikaynakScopus
dc.language.isotr
dc.publisherVeteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBombyx morien_US
dc.subjectGenetic polymorphismen_US
dc.subjectRAPD-PCRen_US
dc.subjectSDS-PAGEen_US
dc.titleMolecular analysis of three local silkworm breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE methodsen_US
dc.title.alternativeÜç yerli i?pekböceği irkının (Alaca, Bursa Beyazı ve Hatay Sarısı) RAPD-PCR ve SDS-PAGE yöntemleri ile moleküler analizien_US
dc.typeArticle

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