Surface modification of polyacrylonitrile film by anchoring conductive polyaniline and determination of uricase adsorption capacity and activity

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorMetin, Ayşegül Ülkü
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2020-06-25T17:49:14Z
dc.date.available2020-06-25T17:49:14Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.description.abstractPolyacrylonitrile (PAN) films were modified with chemical polymerization of conductive polyaniline (PANI) in the presence of potassium dichromate as an oxidizing agent. The effect of aniline concentration on the grafting efficiency and on the electrical surface resistance of PAN and (PAN/PANI)-1-3 composite film was investigated. The surface resistances of the conductive composite films were found to be between 6.32 and 0.97 k Omega/cm. As the amount of grafted PANI increased on the PAN films, the electrical resistance of composite film decreased. The PAN/PANI composite films were also characterized using SEM and FTIR. The changes in the surface properties of the films were characterized by contact angle measurements. As expected, the PAN, PAN/PANI and PAN/PANI-uricase immobilized films, exhibited different contact angle values and surface free energy due to different interactive functional groups of the films. The conductive films were well characterized and used for immobilization of uricase. The amount of adsorbed enzyme increases with the increase of surface concentration of grafted fibrous polyaniline polymer. The maximum amount of immobilized enzyme onto composite film containing 2.4% PANI was about 216 mu g/cm(2) (i.e., PAN/PANI-3). The immobilized uricase was reused 24 times in batch wise assay in a day. Finally, the immobilized uricase enzyme system was successfully fabricated and applied to determine the uric acid level in human serum samples. (C) 2010 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.apsusc.2010.04.077
dc.identifier.endpage6716en_US
dc.identifier.issn0169-4332
dc.identifier.issn1873-5584
dc.identifier.issue22en_US
dc.identifier.scopus2-s2.0-77955654854
dc.identifier.scopusqualityQ1
dc.identifier.startpage6710en_US
dc.identifier.urihttps://doi.org/10.1016/j.apsusc.2010.04.077
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4669
dc.identifier.volume256en_US
dc.identifier.wosWOS:000278908900038
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofApplied Surface Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectConductive polymeren_US
dc.subjectPolyanilineen_US
dc.subjectContact angleen_US
dc.subjectSurface energyen_US
dc.subjectAdsorptionen_US
dc.subjectUricaseen_US
dc.titleSurface modification of polyacrylonitrile film by anchoring conductive polyaniline and determination of uricase adsorption capacity and activityen_US
dc.typeArticle

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