Fibrous polymer-grafted chitosan/clay composite beads as a carrier for immobilization of papain and its usability for mercury elimination

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dc.contributor.authorMetin, Aysegul Ulku
dc.contributor.authorAlver, Erol
dc.date.accessioned2020-06-25T18:16:27Z
dc.date.available2020-06-25T18:16:27Z
dc.date.issued2016
dc.departmentKırıkkale Üniversitesi
dc.description.abstractPapain, which is an industrially important enzyme, has been immobilized on fibrous polymer-modified composite beads, namely poly(methacrylic acid)-grafted chitosan/clay. Characterization studies have been done using FTIR and SEM analysis. Operating parameters such as pH and initial concentration of papain have been varied to obtain the finest papain immobilized polymermodified composite beads. The immobilization capacity of composite beads has been determined as 34.47 +/- 1.18 (n = 3) mg/g. The proteolytic activity of immobilized papain was operated using bovine serum albumin (BSA) and maximum velocity (V-max) and Michaelis-Menten constant (K-m) values of the free and immobilized enzymes were determined using Lineweaver-Burk and Eadie-Hofstee equations. Usability of papain immobilized polymermodified composite beads as adsorbents for the elimination of mercury was investigated. The maximum removal capacity of PIPMC beads has been found to be 4.88 +/- 0.21 mg Hg/g when the initial metal concentration and weight of polymer-modified composite beads were 50 mg/L and 0.04 g at pH 7, respectively. Mercury removal performance of the papain immobilized polymer-modified composite beads was investigated in conjunction with Cu (II), Zn (II) and Cd (II) ions. The mercury adsorption capacity of papain immobilized polymer-modified composite beads was a slight reduction from 1.15 to 0.89 mg/g in presence of multiple metal salts.en_US
dc.description.sponsorshipResearch Fund of Kirikkale UniversityKirikkale University [2015/46]en_US
dc.description.sponsorshipThis work was supported by the Research Fund of Kirikkale University (2015/46).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1007/s00449-016-1590-0
dc.identifier.endpage1149en_US
dc.identifier.issn1615-7591
dc.identifier.issn1615-7605
dc.identifier.issue7en_US
dc.identifier.pmid27013506
dc.identifier.scopus2-s2.0-84961603797
dc.identifier.scopusqualityQ1
dc.identifier.startpage1137en_US
dc.identifier.urihttps://doi.org/10.1007/s00449-016-1590-0
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6533
dc.identifier.volume39en_US
dc.identifier.wosWOS:000378566700011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofBioprocess And Biosystems Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCompositeen_US
dc.subjectGraftingen_US
dc.subjectPapainen_US
dc.subjectEnzymatic activityen_US
dc.subjectMercury removalen_US
dc.titleFibrous polymer-grafted chitosan/clay composite beads as a carrier for immobilization of papain and its usability for mercury eliminationen_US
dc.typeArticle

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