Evaluation of lysozyme adsorptive behaviour of pHEMA-based affinity membranes related to the surface energy and its components to be used in chromatographic fields

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dc.contributor.authorBayramoglu, G
dc.contributor.authorYilmaz, M
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:40:04Z
dc.date.available2020-06-25T17:40:04Z
dc.date.issued2004
dc.departmentKırıkkale Üniversitesi
dc.description.abstractWe have studied the influence of the surface energy and its components of two different dye-ligand immobilised poly(hydroxyethylmethacrylate) (pHEMA) membranes to the adsorption of a protein "lysozyme" from aqueous solutions. For this purpose, macroporous pHEMA membranes were prepared by UV-initiated photo-polymerisation. Two different affinity dye-ligands (i.e. Reactive Brown-10 (RB-10) and Reactive Green-5 (RG-5)) were immobilised onto pHEMA membranes. The adsorption of lysozyme on the affinity membranes was carried out from solutions in the concentration range 0.05-3.00 mg/ml. The measurements of the contact angle for water, glycerol, formamide, diiodomethane (DIM), ethylene glycol and dimethylsulphoxide (DMSO) on plain, both RB-10 and RG-5 immobilised and their lysozyme covered counterpart pHEMA membranes were made. In accordance to the Young equation, the smaller the surface tension of the test liquid, the smaller becomes the contact angle measured on the membrane samples surfaces. The highest contact angles were obtained with water, whereas DMSO gave the lowest contact angles for all the tested membranes. The surface energy parameters of the investigated membranes were calculated from the measured contact angle values, using the mostly used three methods (i.e. the harmonic mean by Wu, the geometric mean by Fowkes and acid-base by van Oss). The adsorption of lysozyme significantly changed both the contact angles and component of surface free energy. It was found that the immobilisation of both dye-ligand increased the lysozyme adsorption capacity of the affinity membranes most significant, although there is not very large difference of surface energy between plain and both dye-ligand immobilised pHEMA membranes. (C) 2004 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.colsurfa.2004.04.072
dc.identifier.endpage21en_US
dc.identifier.issn0927-7757
dc.identifier.issn1873-4359
dc.identifier.issue1-3en_US
dc.identifier.scopus2-s2.0-4143068074
dc.identifier.scopusqualityQ1
dc.identifier.startpage11en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfa.2004.04.072
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3246
dc.identifier.volume243en_US
dc.identifier.wosWOS:000223974600002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofColloids And Surfaces A-Physicochemical And Engineering Aspects
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectdye-liganden_US
dc.subjectlysozymeen_US
dc.subjectadsorptionen_US
dc.subjectcontact angleen_US
dc.subjectsurface energyen_US
dc.titleEvaluation of lysozyme adsorptive behaviour of pHEMA-based affinity membranes related to the surface energy and its components to be used in chromatographic fieldsen_US
dc.typeArticle

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