Covalent immobilization of lipase onto hydrophobic group incorporated poly(2-hydroxyethyl methacrylate) based hydrophilic membrane matrix

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dc.contributor.authorBayramoğlu, G.
dc.contributor.authorKacar, Y.
dc.contributor.authorDenizli, A.
dc.contributor.authorArica, M.Y.
dc.date.accessioned2020-06-25T17:35:04Z
dc.date.available2020-06-25T17:35:04Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, a hydrophobic group containing monomer, 2-methacrylamidophenyalanine (MAPA) was prepared by using methacrylochloride and phenylalanine. Then, poly(2-hydroxyethyl methacrylate-co-methacrylamido-phenlyalanine) (pHEMA-MAPA) membranes were prepared by UV-initiated photopolymerization of HEMA and MAPA in the presence of an initiator alpha-alpha'-azobisisobutyronitrile (AIBN). The lipase was immobilized onto these membranes by covalent bonding through carbodiimide activation. The amount of enzyme loading on the membranes was increased as the MAPA ratio increased in the membrane structure. Immobilization improved the pH stability of the enzyme as well as its temperature stability. Thermal stability was found to increase with immobilization and at 60 degreesC the thermal stability constants were 1.1 x 10(-1) min for free enzyme and 1.2 x 10(-2) min for the immobilized enzyme. The immobilized enzyme activity was found to be quite stable in repeated experiments. (C) 2002 Elsevier Science Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0260-8774(01)00128-5
dc.identifier.endpage374en_US
dc.identifier.issn0260-8774
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0036568473
dc.identifier.scopusqualityQ1
dc.identifier.startpage367en_US
dc.identifier.urihttps://doi.org/10.1016/S0260-8774(01)00128-5
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3007
dc.identifier.volume52en_US
dc.identifier.wosWOS:000175660900008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofJournal Of Food Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpoly(2-hydroxyethyl methacrylate) membraneen_US
dc.subjectphenylala nineen_US
dc.subjectcovalent bondingen_US
dc.subjectenzyme immobilizationen_US
dc.subjectlipaseen_US
dc.titleCovalent immobilization of lipase onto hydrophobic group incorporated poly(2-hydroxyethyl methacrylate) based hydrophilic membrane matrixen_US
dc.typeArticle

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