Immobilization of polyphenol oxidase on carboxymethylcellulose hydrogel beads: preparation and characterization

Basit Öğe Kaydı
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:34:42Z
dc.date.available2020-06-25T17:34:42Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractCarboxymethylcellulose (CMC) beads were prepared by a liquid curing method in the presence of trivalent ferric ions, and epicholorohydrin was covalently attached to the CMC beads. Polyphenol oxidase (PPO) was then covalently immobilized onto CMC beads. The enzyme loading was 603 mu g g(-1) bead and the retained activity of the immobilized enzyme was found to be 44%. The K-m values were 0.65 and 0.87 mM for the free and the immobilized enzyme, and the V-max values were found to be 1890 and 760 U mg(-1) for the free and the immobilized enzyme, respectively. The optimum pH was 6.5 for the free and 7.0 for the immobilized enzyme. The optimum reaction temperature for the free enzyme was 40 degrees C and for the immobilized enzyme was 45 degrees C. Immobilization onto CMC hydrogel beads made PPO more stable to heat and storage, implying that the covalent immobilization imparted higher conformational stability to the enzyme. (C) 2000 Society of Chemical Industry.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage781en_US
dc.identifier.issn0959-8103
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-0034235496
dc.identifier.scopusqualityQ1
dc.identifier.startpage775en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2850
dc.identifier.volume49en_US
dc.identifier.wosWOS:000088071900021
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Ltden_US
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcarboxymethylcellulose beadsen_US
dc.subjectcovalent bondingen_US
dc.subjectenzyme immobilizationen_US
dc.subjectpolyphenol oxidaseen_US
dc.subjectphenolen_US
dc.titleImmobilization of polyphenol oxidase on carboxymethylcellulose hydrogel beads: preparation and characterizationen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Immobilization of polyphenol oxidase on carboxymethylcellulose hydrogel beads preparation and characterization.pdf
Boyut:
150.91 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu