The effects of Ara-C, simvastatin and combo therapy on energy metabolism of HL-60 promyolocytic leukemia cell lines

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dc.contributor.authorBoyunağa H.
dc.contributor.authorGünnur Dikmen Z.
dc.contributor.authorKenar L.
dc.contributor.authorSaygun O.
dc.contributor.authorKeleş H.
dc.contributor.authorUgur Ural A.
dc.contributor.authorLale M.
dc.date.accessioned2020-06-25T15:17:24Z
dc.date.available2020-06-25T15:17:24Z
dc.date.issued2013
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjective: Cancer cells choose their metabolic pathway depending on the oxygen content and substrate concentration of the medium. A wide spectrum of therapeutic agents regulating the energy metabolism of cancer cells are in still in use. Cytosine arabinoside (Ara-C) is a pyrimidine analogue used in the treatment of acute myeloid leukemia (AML) and simvastatin is an inhibitor of HMG-CoA (3-hydroxy-3-methyl-glutaryl-CoA) reductase, which regulates cholesterol biosynthesis. Thus, this study aimed to assess the energy metabolism of HL-60 promyelocytic leukemia cells and healthy white blood cells, additionally to determine the effects of simvastatin and Ara-C, alone or in combination on the energy metabolism of these cells. Materials and Methods: Healthy white blood cells, untreated and treated HL-60 promyolocytic leukemia cell lines were incubated for 4 hours with radiolabelled glucose. Following incubation, lactate, which is one of the end products of the carbohydrate catabolism, and radiolabelled CO2 produced by cells were collected and measured by the liquid scintillation device. In addition, glycogen consumption per hour was determined in each group. Results and Conclusion: We found that untreated HL-60 promyolocytic cells use anaerobic glycolytic pathway whereas healthy white blood cells use aerobic glycolysis for energy gain. It was concluded that combined use of Ara-C and Simvastatin might lead to significant increase in the rate of aerobic glycolysis of HL-60 promyelocytic cells and the metabolism of these leukemia cells become more similar to the metabolism of healthy white blood cells which they originate from. © TurkJBiochem.com.en_US
dc.identifier.citationBoyunağa, H., Dikmen, Z. G., Kenar, L., Saygun, O., Keleş, H., Ural, A. U., Lale, M., Koşargelir, M. (2013). The Effects of Ara-C, Simvastatin and combo therapy on İY energy metabolism of HL-60 promyolocytic leukemia cell lines. Türk Biyokimya Dergisi, 38(1), 31 - 36.en_US
dc.identifier.doi10.5505/tjb.2013.72792
dc.identifier.endpage36en_US
dc.identifier.issn1303829X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84877088081
dc.identifier.scopusqualityQ3
dc.identifier.startpage31en_US
dc.identifier.trdizinid281561
dc.identifier.urihttps://doi.org/10.5505/tjb.2013.72792
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2348
dc.identifier.volume38en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTurkish Biochemistry Societyen_US
dc.relation.ispartofTurkish Journal of Biochemistry
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAra-Cen_US
dc.subjectEnergy metabolismen_US
dc.subjectHL-60 cell linesen_US
dc.subjectSimvastatinen_US
dc.subjectWarburg effecten_US
dc.titleThe effects of Ara-C, simvastatin and combo therapy on energy metabolism of HL-60 promyolocytic leukemia cell linesen_US
dc.title.alternativeHL-60 lösemi hücrelerinde Ara-C ve Simvastatinin tek tek ve kombine kullanımlarının enerji metabolizması üzerindeki etkilerien_US
dc.typeArticle

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