Procion Brown MX-5BR attached and Lewis metals ion-immobilized poly(hydroxyethyl methacrylate)/chitosan IPNs membranes: Their lysozyme adsorption equilibria and kinetics characterization

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dc.contributor.authorBayramoğlu, G.
dc.contributor.authorKaya, B.
dc.contributor.authorArica, M.Y.
dc.date.accessioned2020-06-25T17:35:00Z
dc.date.available2020-06-25T17:35:00Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.description.abstractInterpenetration networks (IPNs) in membrane form were synthesized from 2-hydroxyethyl methacrylate (HEMA) and chitosan (pHEMA/chitosan) via UV-initiated photo-polymerization in the presence of an initiator alpha, alpha'-azoisobutyronitrile. Procion Brown MX-5BR (PB MX-5BR) was covalently attached onto IPNs membrane as a metal chelating dye-ligand. Two different Lewis metal ions (Fe(III) or Cu(II)) were immobilized onto the dye-ligand for utilization in the immobilized metal affinity chromatography (IMAC). The binding characteristics of a model protein (lysozyme) to IMAC adsorbents and selectivity of immobilized metal ions (Fe(III) and Cu(II)) to the lysozyme have been investigated from aqueous solution using the dye-ligand-attached IPNs membrane as a control system. The experimental data was analysed using the two adsorption kinetic models the pseudo-first-order and pseudo-second order to determine the best-fit equation for the adsorption of lysozyme onto dye-ligand and IMAC adsorbents. The second-order equation for the adsorption of lysozyme on the dye-ligand, dye-ligand-Fe(III) and dye-ligand-Cu(II) membrane systems is the most appropriate equation to predict the adsorption capacity for all the tested adsorbents. The reversible lysozyme adsorption on the dye-ligand and IMAC adsorbents obeyed the Temkin isotherm. The lysozyme adsorption capacities of the dye-ligand, dye-ligand-Fe(III) and dye-ligand-Cu(II) immobilized IPNs membranes were 79.1, 147.4, and 128.2 mg ml(-1), respectively. The adsorption of the lysozyme on the pHEMA/chitosan membrane plain was about 8.3 mg ml(-1). (C) 2002 Elsevier Science Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0009-2509(02)00141-0
dc.identifier.endpage2334en_US
dc.identifier.issn0009-2509
dc.identifier.issn1873-4405
dc.identifier.issue13en_US
dc.identifier.scopus2-s2.0-0037135353
dc.identifier.scopusqualityQ1
dc.identifier.startpage2323en_US
dc.identifier.urihttps://doi.org/10.1016/S0009-2509(02)00141-0
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2989
dc.identifier.volume57en_US
dc.identifier.wosWOS:000176996800001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofChemical Engineering Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectadsorptionen_US
dc.subjectpolymersen_US
dc.subjectmembranesen_US
dc.subjectkineticsen_US
dc.subjectIPNsen_US
dc.subjectIMACen_US
dc.subjectlysozymeen_US
dc.titleProcion Brown MX-5BR attached and Lewis metals ion-immobilized poly(hydroxyethyl methacrylate)/chitosan IPNs membranes: Their lysozyme adsorption equilibria and kinetics characterizationen_US
dc.typeArticle

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