Determining Gene Expression Profile of GPX 1 in the Liver of Diabetic Rats Treated with Capsaicin by Real-Time PCR

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dc.contributor.authorKurum, Aytul
dc.contributor.authorKocamis, Hakan
dc.contributor.authorDeprem, Turgay
dc.contributor.authorCinar, Mehmet Ulas
dc.date.accessioned2020-06-25T18:13:33Z
dc.date.available2020-06-25T18:13:33Z
dc.date.issued2015
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe purpose of the present study was to determine the Glutathione Peroxidase 1 (GPX 1) gene expression by Real Time PCR in the liver of healthy and diabetic rats treated with capsaicin. Twenty Sprague-Dawley rats were used in our study. Rats were divided into four groups: Group I: diabetic rats (n=5), Group II: capsaicin injected rats (n=5), Group III: Capsaicin injected diabetic rats (n=5), and Group IV: control rats (n=5). Capsaicin injection began 72 h after streptozotocin (STZ) injection. Capsaicin (1 mg/kg) was prepared in 10% ethanol, 1% Tween 20, and 80% sterile water and subcutaneously injected daily in both Groups II and III for two weeks. The results of RT-PCR conducted to determine GPX 1 gene expression indicated that capsaicin treated diabetic rats had higher GPX 1 expression compared to all groups including control (P<0.05). As a result, capsaicin causes an increase in GPX 1 gene expression at transcription level in the diabetic rat liver. Further investigation is needed to determine if such an increase occurs at protein level using various methods such as western-blot analysis.en_US
dc.description.sponsorshipKirikkale University Scientific Research Coordination UnitKirikkale University [2012/51]en_US
dc.description.sponsorshipThis study was supported by Kirikkale University Scientific Research Coordination Unit (Project no. 2012/51)en_US
dc.identifier.citationKürüm A., Kocamış H., Deprem T., Çınar M. U. (2015). Determining gene expression profile of GPX 1 in the liver of diabetic rats treated with Capsaicin by Real-Time PCR. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(2), 259 - 263.en_US
dc.identifier.doi10.9775/kvfd.2014.12233
dc.identifier.endpage263en_US
dc.identifier.issn1300-6045
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84921807880
dc.identifier.scopusqualityQ3
dc.identifier.startpage259en_US
dc.identifier.trdizinid166847
dc.identifier.urihttps://doi.org/10.9775/kvfd.2014.12233
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6222
dc.identifier.volume21en_US
dc.identifier.wosWOS:000349190400018
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGPX 1en_US
dc.subjectRT-PCRen_US
dc.subjectCapsaicinen_US
dc.subjectDiabetesen_US
dc.subjectLiveren_US
dc.subjectRaten_US
dc.titleDetermining Gene Expression Profile of GPX 1 in the Liver of Diabetic Rats Treated with Capsaicin by Real-Time PCRen_US
dc.typeArticle

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