Inhibitory effect of N-acetyl cysteine and ascorbic acid on the development of myringosclerosis: An experimental study

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dc.contributor.authorDundar, Riza
dc.contributor.authorInan, Sevinc
dc.contributor.authorMuluk, Nuray Bayar
dc.contributor.authorCingi, Cemal
dc.contributor.authorIlknur, Ali Ekber
dc.contributor.authorKatilmis, Huseyin
dc.date.accessioned2020-06-25T18:12:11Z
dc.date.available2020-06-25T18:12:11Z
dc.date.issued2014
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjectives: This study investigated the effects of ascorbic acid and N-acetyl cysteine (NAC) antioxidants on the development of myringosclerosis (MS) in an experimental model. Methods: Myringotomies were performed in the ears of 15 guinea pigs, and Spongostan (R) pieces were placed on the perforated regions of the tympanic membrane. The subjects were divided randomly into three groups and treated with three different solutions on the Spongostan-group 1: (control, 0.9% saline), group 2 (ascorbic acid), and group 3 (NAC). On day 15 after treatment, specimens from the tympanic membranes were obtained and examined via light microscopy. Sclerosis and inflammation scores and the tympanic membrane thicknesses were evaluated. Immunohistochemical methods were used to evaluate the expression of VEGF, TGF-beta, iNOS, and IL1-beta in all groups. Results: Lower sclerosis and inflammation scores and reduced tympanic membrane thicknesses were observed in groups treated with NAC or ascorbic acid compared with the control group. Immunohistochemical studies revealed significantly less expression of VEGF, TGF-beta, and iNOS in groups 2 and 3 compared with group 1. Additionally, IL1-beta expression was significantly less in group 3 than in group 1. Compared with group 1, group 2 animals exhibited reduced inflammation in the lamina propria, fewer active fibroblasts, less leukocyte infiltration, and decreased thickness of the vessels; group 3 animals exhibited decreased numbers of active fibroblasts and collagen fibers in the lamina propria. Conclusions: Inflammation scores, cellular infiltration, and expression of VEGF, TGF-beta, and iNOS were reduced by ascorbic acid and/or NAC treatments, thereby decreasing MS development. Decreased expression of IL1-beta was observed only in animals treated with NAC. (C) 2014 Elsevier Ireland Ltd. All rights reserved.en_US
dc.description.sponsorshipContinuous Education and Scientific Research Associationen_US
dc.description.sponsorshipWith the exception of data collection, the preparation of this paper, including design and planning, was supported by the Continuous Education and Scientific Research Association.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.ijporl.2014.03.029
dc.identifier.endpage1025en_US
dc.identifier.issn0165-5876
dc.identifier.issn1872-8464
dc.identifier.issue7en_US
dc.identifier.pmid24794623
dc.identifier.scopus2-s2.0-84902082179
dc.identifier.scopusqualityQ2
dc.identifier.startpage1019en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijporl.2014.03.029
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5785
dc.identifier.volume78en_US
dc.identifier.wosWOS:000338608100010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofInternational Journal Of Pediatric Otorhinolaryngology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAscorbic aciden_US
dc.subjectInducible nitric oxide synthase (iNOS)en_US
dc.subjectInterleukin 1-beta (IL-beta)en_US
dc.subjectMyringosclerosisen_US
dc.subjectN-acetyl cysteine (NAC)en_US
dc.subjectVascular endothelial growth factor (VEGF)en_US
dc.titleInhibitory effect of N-acetyl cysteine and ascorbic acid on the development of myringosclerosis: An experimental studyen_US
dc.typeArticle

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