Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus

Basit Öğe Kaydı
dc.contributor.authorAzkur, Ahmet Kursat
dc.contributor.authorvan der Poel, Wim H. M.
dc.contributor.authorAksoy, Emel
dc.contributor.authorHakze-van der Honing, Renate
dc.contributor.authorYildirim, Murat
dc.contributor.authorYildiz, Kader
dc.date.accessioned2021-01-14T18:10:27Z
dc.date.available2021-01-14T18:10:27Z
dc.date.issued2020
dc.departmentKKÜ
dc.description.abstractSchmallenberg virus (SBV), discovered in Germany in 2011, causes congenital malformations in ruminants. Reverse-transcription real-time PCR (RT-rtPCR) assays based on various segments of SBV have been developed for molecular detection. We developed alternative RT-rtPCR assays for SBV detection to avoid earlier reported mutations and hypervariable regions of the S and M segments of the viral genome. For SYBR Green-based detection of the S segment, theR(2)value and efficiency of the developed assay were 0.99 and 99%, respectively. For probe-based S segment detection, 2 assays were developed; the first had anR(2)value of 0.99 and 102% efficiency, and the second had aR(2)value of 0.98 and 86% efficiency. The probe-based M segment assay had anR(2)value of 1.00 and 103% efficiency. Detection limits of the RT-rtPCR assays with new primer sets were 10(2)and 10(1)copies/mu L for the S and M segments, respectively. Field samples from cattle and sheep were also used for primary validation of the developed assays. Our assays should be suitable for SBV detection in ruminants and for in vitro studies of various SBV strains.en_US
dc.description.sponsorshipGeneral Directorate of Agricultural Research and Policies, Ministry of Agriculture and Forestry, Republic of Turkey [TAGEM15/AR-GE48]en_US
dc.description.sponsorshipThis study is a part of a project supported by the General Directorate of Agricultural Research and Policies, Ministry of Agriculture and Forestry, Republic of Turkey (grant TAGEM15/AR-GE48).en_US
dc.identifier.citationBu makale açık erişimli değildir.en_US
dc.identifier.doi10.1177/1040638720947199
dc.identifier.endpage717en_US
dc.identifier.issn1040-6387
dc.identifier.issn1943-4936
dc.identifier.issue5en_US
dc.identifier.pmid32757829
dc.identifier.scopus2-s2.0-85089177434
dc.identifier.scopusqualityQ1
dc.identifier.startpage710en_US
dc.identifier.urihttps://doi.org/10.1177/1040638720947199
dc.identifier.urihttps://hdl.handle.net/20.500.12587/12606
dc.identifier.volume32en_US
dc.identifier.wosWOS:000556848300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSAGE PUBLICATIONS INCen_US
dc.relation.ispartofJOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectM segmenten_US
dc.subjectRT-rtPCRen_US
dc.subjectS segmenten_US
dc.subjectSchmallenberg virusen_US
dc.subjectSYBR Greenen_US
dc.titleDevelopment and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virusen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus.pdf
Boyut:
911.01 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu