Phenotypic and genotypic antibiotic resistance profiles of Escherichia coli O157 from cattle and slaughterhouse wastewater isolates

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dc.contributor.authorAyaz, Naim Deniz
dc.contributor.authorGencay, Yilmaz Emre
dc.contributor.authorErol, Irfan
dc.date.accessioned2020-06-25T18:13:13Z
dc.date.available2020-06-25T18:13:13Z
dc.date.issued2015
dc.departmentKırıkkale Üniversitesi
dc.descriptionGencay, Yilmaz Emre/0000-0002-2154-9663
dc.description.abstractThe aims of this study were to determine the minimal inhibition concentration of 20 different antibiotics on cattle and slaughterhouse wastewater Escherichia coli O157, including both Shiga toxigenic E. coli O157 (STEC O157) and non-Shiga toxigenic strains (non-STEC O157) by the Epsilometer test, and to determine the antibiotic resistance gene profiles of the isolates by PCR. A total of 102 cattle and slaughterhouse wastewater E. coli O157 isolates including 96 E. coli O157:H7(+) (81 non-sorbitol fermenting [NSF] STEC O157:H7, 12 NSF non-STEC O157:H7, and three sorbitol fermenting [SF] non-STEC O157:H7) and six non-STEC O157:H7(-) isolated from 744 cattle and slaughterhouse wastewater samples collected within a 2-year period were assessed. Of 93 NSF E. coli O157:H7 isolates, 19 were resistant to tetracycline and sulfamethoxazole, 14 to trimethoprim, 13 to cefoxitin, 11 to streptomycin, 10 to ampicillin, eight to chloramphenicol, six to cephalothin, four to cefaclor, four to aztreonam, and four to nalidixic acid. In six of the E. coli O157:H7(-) isolates, tetracycline resistance was detected while five of them were also resistant to ampicillin, sulfamethoxazole, and trimethoprim. In PCR analysis, 26.0 % (25/96) of the NSF E. coli O157:H7(+) and all of the E. coli O157:H7(-) isolates harbored one or more antibiotic resistance genes. While tetA, tetB, tetC, strA, strB, and sulI genes were detected from a number of the isolates, tetD, tetE, tetG, cmlA, floR, sulII, aadA, and ampC genes were not detected in any of the isolates. Results suggest a high antibiotic resistance in E. coli O157:H7(+)/H7(-) cattle and wastewater isolates. The majority of our resistant isolates, antibacterial resistance genes did not correlate with observed phenotypic resistance. Other resistance traits and regulatory factors that mediate antibiotic resistance should be included in further antimicrobial resistance investigations.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110R013]en_US
dc.description.sponsorshipThis study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, project no: 110R013).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1007/s13213-014-0961-5
dc.identifier.endpage1144en_US
dc.identifier.issn1590-4261
dc.identifier.issn1869-2044
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85027946528
dc.identifier.scopusqualityQ2
dc.identifier.startpage1137en_US
dc.identifier.urihttps://doi.org/10.1007/s13213-014-0961-5
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6153
dc.identifier.volume65en_US
dc.identifier.wosWOS:000354724300054
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofAnnals Of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectE. coli O157:H7en_US
dc.subjectAntibiotic resistanceen_US
dc.subjectMICen_US
dc.subjectE-Testen_US
dc.subjectResistance genesen_US
dc.subjectPCRen_US
dc.titlePhenotypic and genotypic antibiotic resistance profiles of Escherichia coli O157 from cattle and slaughterhouse wastewater isolatesen_US
dc.typeArticle

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