A disposable gold-cellulose nanofibril platform for SERS mapping

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dc.contributor.authorTanis, Saliha Nur
dc.contributor.authorİlhan, Hasan
dc.contributor.authorGüven, Burcu
dc.contributor.authorTayyarcan, Emine Kübra
dc.contributor.authorÇiftçi, Hakan
dc.contributor.authorSaglam, Necdet
dc.contributor.authorHakki Boyaci, Ismail
dc.date.accessioned2021-01-14T18:10:33Z
dc.date.available2021-01-14T18:10:33Z
dc.date.issued2020
dc.departmentKKÜ
dc.descriptionTayyarcan, Emine Kubra/0000-0002-5765-3884; Tamer, Ugur/0000-0001-9989-6123; Ilhan, Hasan/0000-0002-4475-1629
dc.description.abstractIn this study, we present a disposable and inexpensive paper-like gold nanoparticle-embedded cellulose nanofibril substrate for the rapid enumeration ofEscherichia coli(E. coli) using surface-enhanced Raman scattering (SERS) mapping. A disposable SERS substrate was simply constructed by mixing CNF and gold chloride solution at 120 degrees C in a water bath. The application of the resulting substrate was carried out by enrichment and SERS detection ofE. coli. To this end, the spherical gold nanoparticle-embedded cellulose nanofibril substrate was used as a scavenger forE. coli. After the target bacteriaE. coliwere separated from the matrixviaoriented antibodies, the sandwich assay procedure was carried out using 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB)-coated Au nanorod particles that acted as SERS mapping probes. The distribution density of DTNB was demonstrated visually using SERS mapping, and the assay was completed in one hour. The correlation between theE. coliand SERS mapping signals was found to be linear within the range of 15 cfu mL(-1)to 1.5 x 10(5)cfu mL(-1). The limit of detection for the SERS mapping assay was determined to be 2 cfu mL(-1). The selectivity of the developed method was examined withMicrococcus luteus(M. luteus),Bacillus subtilis(B. subtilis), andEnterobacter aerogenes(E. aerogenes), which did not produce any significant response. Furthermore, the developed method was evaluated for detectingE. coliin artificially contaminated samples, and the results were compared with those of the plate-counting method.en_US
dc.description.sponsorshipGazi University BAP ProjectGazi University [02/2018-08]en_US
dc.description.sponsorshipThe authors acknowledge Gazi University BAP Project no: 02/2018-08 for the financial support.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1039/d0ay00662a
dc.identifier.endpage3172en_US
dc.identifier.issn1759-9660
dc.identifier.issn1759-9679
dc.identifier.issue24en_US
dc.identifier.pmid32930178
dc.identifier.scopus2-s2.0-85087278098
dc.identifier.scopusqualityQ1
dc.identifier.startpage3164en_US
dc.identifier.urihttps://doi.org/10.1039/d0ay00662a
dc.identifier.urihttps://hdl.handle.net/20.500.12587/12664
dc.identifier.volume12en_US
dc.identifier.wosWOS:000545442100010
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherROYAL SOC CHEMISTRYen_US
dc.relation.ispartofANALYTICAL METHODS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleA disposable gold-cellulose nanofibril platform for SERS mappingen_US
dc.typeArticle

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