Designing of a stable and selective glucose biosensor by glucose oxidase immobilization on glassy carbon electrode sensitive to H2O2 via nanofiber interface
Yükleniyor...
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SPRINGER
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The integration of the enzymes on the solid electrode surfaces is an indispensable step for the construction of the bioelectrochemical electrode. In the current work, the blend nanofibers consisting of poly (vinyl alcohol) and poly(ethyleneimine) were deposited by the electrospinning method on an H2O2-sensitive modified glassy carbon electrode. Glucose oxidase was immobilized on the glutaraldehyde-activated blend nanofibers. Bioelectrochemical electrode displayed a good linear response to the glucose concentration ranges with two separate calibration curves, from 2 to 8 mmol L-1 and from 10 to 30 mmol L-1. Besides, it showed a high anti-interference performance against ascorbic and uric acids as well as long-term storage stability over 63 days. Moreover, analysis results in a diluted human serum sample showed that the prepared bioelectrochemical electrode has the ability to measure glucose in real samples.
Açıklama
Temocin, Zulfikar/0000-0001-7151-9772
Anahtar Kelimeler
Glassy carbon, Glucose oxidase, Nanofiber, Glucose biosensor, Poly(vinyl alcohol)
Kaynak
JOURNAL OF APPLIED ELECTROCHEMISTRY
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
Sayı
Künye
closedAccess
