β-galactosidase immobilization into poly(hydroxyethyl methacrylate) membrane and performance in a continuous system

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dc.contributor.authorArica, MY
dc.contributor.authorBaran, T
dc.contributor.authorDenizli, A
dc.date.accessioned2020-06-25T17:34:39Z
dc.date.available2020-06-25T17:34:39Z
dc.date.issued1999
dc.departmentKırıkkale Üniversitesi
dc.descriptionBaran, Erkan/0000-0002-0563-6943; Baran, Erkan/0000-0002-0563-6943;
dc.description.abstractThe activity of beta-galactosidase immobilized into a poly(2-hydroxyethyl methacrylate) (pHEMA) membrane increased from 1.5 to 10.8 U/g pHEMA upon increase in enzyme loading. The K-m values for the free and the entrapped enzyme were found to be 0.26 and 0.81 mM, respectively. The optimum reaction temperatures for the free and the entrapped beta-galactosidase were both found to be 50 degrees C. Similarly, the optimum reaction pH was 7.5 for both the free and the entrapped enzyme. The immobilized beta-galactosidase was characterized in a continuous system during lactose hydrolysis and the operational inactivation rate constant (k(iop)) of the entrapped enzyme was found to be 3.1 x 10(-5) min(-1). (C) 1999 John Wiley & Sons, Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/(SICI)1097-4628(19990606)72:10<1367
dc.identifier.endpage1373en_US
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue10en_US
dc.identifier.scopus2-s2.0-0032687636
dc.identifier.scopusqualityQ2
dc.identifier.startpage1367en_US
dc.identifier.urihttps://doi.org/10.1002/(SICI)1097-4628(19990606)72:10<1367
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2822
dc.identifier.volume72en_US
dc.identifier.wosWOS:000079480800017
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofJournal Of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbeta-galactosidaseen_US
dc.subjectenzyme immobilizationen_US
dc.subjectpHEMAen_US
dc.subjectlactose hydrolysisen_US
dc.subjectenzyme-membrane reactoren_US
dc.titleβ-galactosidase immobilization into poly(hydroxyethyl methacrylate) membrane and performance in a continuous systemen_US
dc.typeArticle

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