Cytochrome c adsorption on glutamic acid ligand immobilized magnetic poly(methylmethacrylate-co-glycidylmethacrylate) beads

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorLoğoğlu, Elif
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2020-06-25T17:43:50Z
dc.date.available2020-06-25T17:43:50Z
dc.date.issued2007
dc.description.abstractThis work presents data on cytochrome c adsorption onto glutamic acid immobilized magnetic poly(methylmethacrylate-coglycidylmethacrylate), mp(GMA-MMA) beads which were synthesized from glycidylmethacrylate (GMA) and methylmethacrylate (MMA) in the presence of a cross-linker (i.e., ethyleneglycol dimethacrylate; EGDMA) via suspension polymerization. The epoxy groups of the mp(GMA-MMA) beads were converted into amino groups after reaction with ammonia and the aminated magnetic beads was activated with glutaric dialdhyde. It was then glutamic acid as an amino acid ligand covalently immobilized on the activated beads. The affinity mp(GMA-MMA)-A-GA beads were used in cytochrome c (Cytc) adsorption studies under defined pH, ionic strength or temperature conditions in a batch system using plain mp(GMA-MMA)-A beads as a control system. The maximum adsorption capacity of the mp(GMA-MMA)-A-GA affinity beads was found to be 140.3 mg g(-1) beads and the affinity constant (K-d), evaluated by the Langmuir model, was 5.42 x 10(-6) M. Adsorption capacity of the mp(GMA-MMA)-A-GA were decreased to Cytc by increasing the ionic strength adjusted with NaCl. Adsorption kinetic of Cytc onto magnetic affinity beads was analyzed with first-order and second-order kinetic equations. The first-order equation fitted well with the experimental data. (c) 2006 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.colsurfa.2006.10.023
dc.identifier.endpage62en_US
dc.identifier.issn0927-7757
dc.identifier.issn1873-4359
dc.identifier.issue1-3en_US
dc.identifier.scopus2-s2.0-33847060797
dc.identifier.scopusqualityQ1
dc.identifier.startpage55en_US
dc.identifier.urihttps://doi.org10.1016/j.colsurfa.2006.10.023
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3921
dc.identifier.volume297en_US
dc.identifier.wosWOS:000245072500008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofColloids And Surfaces A-Physicochemical And Engineering Aspects
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmagnetic beadsen_US
dc.subjectaffinity chromatographyen_US
dc.subjectadsorptionen_US
dc.subjectL-glutamic aciden_US
dc.subjectcytochrome cen_US
dc.titleCytochrome c adsorption on glutamic acid ligand immobilized magnetic poly(methylmethacrylate-co-glycidylmethacrylate) beadsen_US
dc.typeArticle

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