Schmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manner

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dc.contributor.authorAksoy, Emel
dc.contributor.authorAzkur, Ahmet Kursat
dc.date.accessioned2020-06-25T18:34:20Z
dc.date.available2020-06-25T18:34:20Z
dc.date.issued2019
dc.departmentKırıkkale Üniversitesi
dc.descriptionAKSOY, Emel/0000-0001-9536-4322
dc.description.abstractSchmallenberg virus (SBV), discovered in 2011 in Germany, is associated with clinical manifestations of fever, diarrhea, reduced milk yield, abortions and congenital malformations in ruminants. Despite many studies performed for SBV, there is no detailed research on in vitro apoptotic effect of SBV. This study is aimed to determine apoptosis pathways and role of proapoptotic and anti-apoptotic molecules in Vero cells infected with SBV. The study results showed that SBV induced apoptosis via both extrinsic and intrinsic pathways by activating both caspase-8 and caspase-9, respectively. Expression analyses of pro-apoptotic (Bax, Bak and Puma) and anti-apoptotic (Bcl-2 and Bcl-XL) genes revealed that SBV-induced apoptosis causes upregulation of pro-apoptotic genes, dominantly via Puma gene, whereas Bcl-2 and Bcl-XL genes were downregulated. In conclusion, this is the first detailed report about SBV induced apoptosis in the Vero cells via both extrinsic and intrinsic cascades and apoptosis induction is seem to be regulated by Puma.en_US
dc.description.sponsorshipScientific Research Project Coordination Unit (BAP) of Kirikkale University [KU-BAP 2015/052]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Project Coordination Unit (BAP) of Kirikkale University [grant number KU-BAP 2015/052] and includes part of PhD dissertation of Emel AKSOY. The authors would also like to thank Prof. Dr. Latif OZTURK (Kirikkale University, Faculty of Economics and Administrative Sciences, Department of Econometrics, Kirikkale, Turkey) for his advice and help in statistical analysis.en_US
dc.identifier.citationAksoy E, Azkur AK. Schmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manner. J Vet Med Sci. 2019.9;81(2):204-212.en_US
dc.identifier.doi10.1292/jvms.18-0582
dc.identifier.endpage212en_US
dc.identifier.issn0916-7250
dc.identifier.issn1347-7439
dc.identifier.issue2en_US
dc.identifier.pmid30541984
dc.identifier.scopus2-s2.0-85061385083
dc.identifier.scopusqualityQ2
dc.identifier.startpage204en_US
dc.identifier.urihttps://doi.org/10.1292/jvms.18-0582
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7869
dc.identifier.volume81en_US
dc.identifier.wosWOS:000459994300010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherJapan Soc Vet Scien_US
dc.relation.ispartofJournal Of Veterinary Medical Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectapoptosisen_US
dc.subjectcaspaseen_US
dc.subjectDNA fragmentationen_US
dc.subjectflow cytometryen_US
dc.subjectSchmallenberg virusen_US
dc.titleSchmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manneren_US
dc.typeArticle

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