Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: a simple technique

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dc.contributor.authorNiyaz, Mehmet
dc.contributor.authorGurpinar, Ozer Aylin
dc.contributor.authorGunaydin, Serdar
dc.contributor.authorOnur, Mehmet Ali
dc.date.accessioned2020-06-25T18:06:53Z
dc.date.available2020-06-25T18:06:53Z
dc.date.issued2012
dc.departmentKırıkkale Üniversitesi
dc.descriptionONUR, MEHMET/0000-0002-3630-7982
dc.description.abstractIn this study, our aim was to develop a new simple technique for isolation of mesenchymal stem cells from adipose tissue. For this purpose, mesenchymal stem cells were isolated from rat adipose tissue by using the primary explant culture technique. When the cells became confluent, they were passaged 4 times by using the standard trypsinization method with trypsin/EDTA solution. Cells at second passage were characterized by using immunofluorescence staining against CD13 and CD29 markers. The results showed that these cultured cells were positive for CD13 and CD29 markers. Flow cytometry analysis was also done against CD29, CD90, CD54, MHC Class I, CD45, CD 106, and MHC Class II for characterization of mesenchymal stem cells. The results of flow cytometry analysis showed that these cells were mesenchymal stem cells. Half of the cells were cryopreserved at all passages for future applications. It is thought that these mesenchymal stem cells can be used in therapy of cardiovascular diseases as an alternative technique in the near future.en_US
dc.description.sponsorshipKirikkale UniversityKirikkale University [2010/15]en_US
dc.description.sponsorshipThis study was supported by the Kirikkale University Research Fund (Project Number: 2010/15). We also thank Asim Niyaz for his support with the revision of the article.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.3906/biy-1109-31
dc.identifier.endpage664en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-84868564854
dc.identifier.scopusqualityQ1
dc.identifier.startpage658en_US
dc.identifier.trdizinid137444
dc.identifier.urihttps://doi.org/10.3906/biy-1109-31
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5397
dc.identifier.volume36en_US
dc.identifier.wosWOS:000312424500006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdipose tissueen_US
dc.subjectmesenchymal stem cellen_US
dc.subjectCD13en_US
dc.subjectCD29en_US
dc.subjectCD90en_US
dc.subjectCD45en_US
dc.subjectCD54en_US
dc.subjectMHC Class Ien_US
dc.subjectMHC Class IIen_US
dc.subjectimmunofluorescence stainingen_US
dc.subjectcryopreservationen_US
dc.titleIsolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: a simple techniqueen_US
dc.typeArticle

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