A safety investigation into topical effects of naproxen sodium on nasal epithelial cells and potential toxicity in local application

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dc.authoridCingi, Cemal/0000-0003-3934-5092
dc.authoridBAYAR MULUK, NURAY/0000-0003-3602-9289
dc.authoridKutlu, Mehtap/0000-0002-8816-1487
dc.authoridKaya, Furkan/0000-0001-7216-5187
dc.contributor.authorDundar, Riza
dc.contributor.authorMuluk, Nuray Bayar
dc.contributor.authorSezer, Canan Vejselova
dc.contributor.authorKaya, Furkan
dc.contributor.authorKutlu, Hatice Mehtap
dc.contributor.authorCingi, Cemal
dc.date.accessioned2025-01-21T16:35:24Z
dc.date.available2025-01-21T16:35:24Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjectives: We examined how topically-applied naproxen sodium affects human nasal epitheliocytes in culture. Methods: Samples of healthy human primary nasal epithelium (NE) harvested during septoplasty from volunteers without rhinosinusitis were incubated in cell culture. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays may be utilised when assessing cellular damage (toxicity), as evidenced by DNA fragmentation, nuclear condensation, alteration in the outer plasma membrane and cytoskeletal alteration. This was the method used in the study. Cultured epitheliocytes were incubated with naproxen sodium for 24 h at 37 ???C. The MTT assay was then performed and the cells' morphology was examined by confocal microscopy. Additionally, cellular proliferation was assessed by the artificial scratch method followed by light microscopy. Results: The results indicated that naproxen sodium does not cause any cytotoxic effects upon nasal epithelial cells when applied topically. There was no evidence indicating cytotoxicity on the nasal epitheliocytes in culture for the 24 h period over which the drug was applied. In particular, there was no alteration in cellular morphology, damage to the intracellular organelles structure or the cytoskeleton secondary to naproxen sodium. Furthermore, cellular proliferation occurred normally in these conditions, as on scratch test. Conclusion: Topical naproxen sodium may be used on nasal epithelial cells without inducing toxicity. This agent is therefore suitable, given its known anti-inflammatory effects, for use in patients suffering from diseases involving nasal and paranasal sinusal inflammation, including rhinosinusitis (both chronic and acute) and nasal polyposis which should be investigated. In the future, topical medication forms for nasal usage should be developed.
dc.identifier.doi10.1016/j.amjoto.2022.103689
dc.identifier.issn0196-0709
dc.identifier.issn1532-818X
dc.identifier.issue1
dc.identifier.pmid36427392
dc.identifier.scopus2-s2.0-85142457879
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.amjoto.2022.103689
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24113
dc.identifier.volume44
dc.identifier.wosWOS:000892329600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherW B Saunders Co-Elsevier Inc
dc.relation.ispartofAmerican Journal of Otolaryngology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectNaproxen sodium; Cultured human nasal epithelial cells; Confocal microscopy; Viability; Toxicity
dc.titleA safety investigation into topical effects of naproxen sodium on nasal epithelial cells and potential toxicity in local application
dc.typeArticle

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