Immobilization of glucoamylase onto activated pHEMA/EGDMA microspheres: properties and application to a packed-bed reactor

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dc.contributor.authorArica, MY
dc.contributor.authorAlaeddinoglu, NG
dc.contributor.authorHasirci, V
dc.date.accessioned2020-06-25T17:34:37Z
dc.date.available2020-06-25T17:34:37Z
dc.date.issued1998
dc.departmentKırıkkale Üniversitesi
dc.description.abstractGlucoamylase was covalently immobilized onto pHEMA/EGDMA microspheres of two different sizes: 50-100 mu m and 100-200 mu m in diameter. The activity of the enzyme on smaller microspheres was found to be almost twice that of the larger microspheres. A higher enzyme lending was observed on small microspheres (0.64 mg g(-1) support) as compared to large spheres (0.40 mg g(-1) support). The K-m of glucoamylase was significantly increased (approximately five times) upon immobilization, indicating decreased affinity by the enzyme for its substrate. V-max of the enzyme was, however, not as significantly altered upon immobilization as the K-m. More significantly, the V-max was much higher with the large substrate (dextrin) than it was with the small substrate (maltose). Activity of the immobilized enzyme was quite stable. In 120 h, only 9.0% of the immobilized glucoamylase activity was lost. (C) 1998 Elsevier Science Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0141-0229(97)00139-7
dc.identifier.endpage157en_US
dc.identifier.issn0141-0229
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0032520206
dc.identifier.scopusqualityQ1
dc.identifier.startpage152en_US
dc.identifier.urihttps://doi.org/10.1016/S0141-0229(97)00139-7
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2806
dc.identifier.volume22en_US
dc.identifier.wosWOS:000071652100003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Incen_US
dc.relation.ispartofEnzyme And Microbial Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpHEMA/EGDMA microspheresen_US
dc.subjectcovalent bondingen_US
dc.subjectenzyme immobilizationen_US
dc.subjectglucoamylaseen_US
dc.subjectenzyme reactoren_US
dc.titleImmobilization of glucoamylase onto activated pHEMA/EGDMA microspheres: properties and application to a packed-bed reactoren_US
dc.typeArticle

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