IMPACTS OF SPECIFIC CRYOPROTECTANTS ON SPERM FREEZING AND RELATIONSHIPS BETWEEN CRYODAMAGE AND OXIDATION STRESS PARAMETERS IN AWASSI RAM SPERM

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dc.contributor.authorVarisli, Omer
dc.contributor.authorErat, Serkan
dc.contributor.authorBozkaya, Faruk
dc.contributor.authorAydilek, Nurettin
dc.contributor.authorTaskin, Abdullah
dc.date.accessioned2025-01-21T16:41:41Z
dc.date.available2025-01-21T16:41:41Z
dc.date.issued2021
dc.departmentKırıkkale Üniversitesi
dc.description.abstractBACKGROUND: The role of oxidative stress during cryoprotectant treatment has received little attention. OBJECTIVE: To assess the effects of different cryoprotectants and discover relationships between cryodamage and oxidative stress parameters on Awassi ram sperm. MATERIALS AND METHODS: The sperm samples diluted with Salamon's tris-citrate (TRIS) containing 20% centrifuged egg yolk and 0.5, 1.0 or 1.5 M Glycerol (Gly), methanol (M), 2-methoxyethanol (2-ME), dimethylacetamide (DMA) and 1.2 propanediol (PR). After 2 h of equilibration at +4 degrees C, the sperm samples were frozen in liquid nitrogen vapour and stored. RESULTS: The best post-thaw motility (43.3%, 41.7%) of sperm was achieved when protected with 0.5 and 1.0 M glycerol. Arylesterase and ceruloplasmin parameters were significantly different after equilibration, whereas sulfhydryl groups were significantly different after freezing in their respective groups (P< 0.05). CONCLUSION: The increased use of glycerol caused greater loss of motility. The role of oxidative stress in freezing was also found to be limited.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [112O911]
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) grant number 112O911. I would like to thank my fellow graduate students for their help.
dc.identifier.endpage95
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue2
dc.identifier.pmid33970985
dc.identifier.scopus2-s2.0-85105723114
dc.identifier.scopusqualityQ3
dc.identifier.startpage87
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24923
dc.identifier.volume42
dc.identifier.wosWOS:000636820500005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCryo Letters
dc.relation.ispartofCryoletters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectAwassi; cryopreservation; cryoprotectants; oxidative stress; ram; sperm
dc.titleIMPACTS OF SPECIFIC CRYOPROTECTANTS ON SPERM FREEZING AND RELATIONSHIPS BETWEEN CRYODAMAGE AND OXIDATION STRESS PARAMETERS IN AWASSI RAM SPERM
dc.typeArticle

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