Evaluation of the effects of thymoquinone on red blood cell deformability, morphology, and endothelial nitric oxide synthase (eNOS) synthesis in rat lower extremity ischemia-reperfusion injury

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dc.contributor.authorGunay, Celalettin
dc.contributor.authorKartal, Hakan
dc.contributor.authorDemirdas, Ertan
dc.contributor.authorOz, Bilgehan Savas
dc.contributor.authorComu, Faruk Metin
dc.contributor.authorErol, Gokhan
dc.contributor.authorArslan, Gokhan
dc.date.accessioned2025-01-21T16:40:45Z
dc.date.available2025-01-21T16:40:45Z
dc.date.issued2024
dc.departmentKırıkkale Üniversitesi
dc.description.abstractBACKGROUND: Erythrocyte deformability refers to the ability of erythrocytes to bend and twist as they pass through capillaries, which is crucial for tissue perfusion. This study aims to investigate the effects of Thymoquinone treatment on erythrocyte deform- ability in rats subjected to lower extremity ischemia-reperfusion injury. METHODS: The study was conducted on Wistar albino rats weighing 400-450 g. The rats were randomly divided into five groups: the control group (C), in which no treatment was applied; the group that received dimethyl sulfoxide (DMSO) as a solvent; the group subjected to 90 minutes of ischemia followed by 90 minutes of reperfusion in the main femoral artery of the lower extremity (IR); the Thymoquinone control group (TQ-C), in which the effects of Thymoquinone alone were examined; and the group that received intraperitoneal Thymoquinone one hour before the ischemia-reper fusion procedure (IR+TQ). At the end of the procedure, intracardiac blood was collected from the rats, and May-Grunwald and Giemsa (MGG) staining, endothelial nitric oxide synthase (eNOS), and erythrocyte deformability indexes were measured. RESULTS: The study results showed significant differences. Erythrocyte deformability was statistically significantly improved in the group that received Thymoquinone before ischemia-reper fusion compared to the group subjected to ischemia-reper fusion only. Morphological changes in erythrocytes were also statistically significantly better in the IR+TQ group than in the IR group. Immunohistochemical eNOS staining revealed that eNOS activity in the IR group was lower than in the IR+TQ group. CONCLUSION: Our study demonstrates that Thymoquinone treatment administered before ischemia exerts protective effects against erythrocyte deformation and morphological deterioration by increasing eNOS activity.
dc.identifier.doi10.14744/tjtes.2024.94055
dc.identifier.endpage721
dc.identifier.issn1306-696X
dc.identifier.issn1307-7945
dc.identifier.issue10
dc.identifier.pmid39382366
dc.identifier.scopus2-s2.0-85206041409
dc.identifier.scopusqualityQ3
dc.identifier.startpage715
dc.identifier.urihttps://doi.org/10.14744/tjtes.2024.94055
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24760
dc.identifier.volume30
dc.identifier.wosWOS:001335981500003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Assoc Trauma Emergency Surgery
dc.relation.ispartofUlusal Travma Ve Acil Cerrahi Dergisi-Turkish Journal of Trauma & Emergency Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectErythrocyte deformability; ischemia-reperfusion; endothelial nitric oxide synthase (eNOS); thymoquinone
dc.titleEvaluation of the effects of thymoquinone on red blood cell deformability, morphology, and endothelial nitric oxide synthase (eNOS) synthesis in rat lower extremity ischemia-reperfusion injury
dc.title.alternativeSıçan alt ekstremite iskemi-reperfüzyon hasarında timokinonun kırmızı kan hücresi deforme edilebilirliği, morfolojisi ve eNOS sentezi üzerine etkilerinin değerlendirilmesi
dc.typeArticle

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