Extracellular traps development in canine neutrophils induced by infective stage Toxocara canis larvae

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dc.contributor.authorAkkus, Gozde Nur
dc.contributor.authorYildiz, Kader
dc.date.accessioned2025-01-21T16:41:07Z
dc.date.available2025-01-21T16:41:07Z
dc.date.issued2024
dc.departmentKırıkkale Üniversitesi
dc.description.abstractNeutrophils, a crucial element of the host defense system, develop extracellular traps against helminth parasites. Neutrophils accumulate around the larvae of Toxocara canis (T. canis) in the tissues of the organism. This study aimed to determine the reaction in canine neutrophils after incubation with infective stage T. canis larvae (L3) in vitro. Most L3 were still active and moved between the extracellular traps (NETs) after 60-min incubation. NETs were not disintegrated by L3 movement. The L3 was only immobilized by NETs, entrapped larvae were still motile between the traps at the 24 h incubation. NETs were observed not only to accumulate around the mouth, excretory pole or anus but also the entire body of live L3. The extracellular DNA amount released from the canine neutrophils after being induced with phorbol 12-myristate 13-acetate was not affected by T. canis excretory/ secretory products obtained from 250 L3. To the Authors'knowledge, the extracellular trap structures was firstly observed in canine neutrophils against T. canis L3 in vitro. NETs decorated with myeloperoxidase, neutrophil elastase and histone (H3) were observed under fluorescence microscope. There were not significant differences in the amount of extracellular DNA (P > 0.05), but the morphological structure of NETs was different in the live and head-inactivated T. canis larvae.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Kirikkale University [2022/090]; Scientific and Technological Research Council of Turkiye [TOVAG] [123O154]
dc.description.sponsorshipThis work was financially supported by Scientific Research Projects Coordination Unit of Kirikkale University [2022/090] and The Scientific and Technological Research Council of Turkiye [TOVAG, 123O154] https:// www.tubitak.gov.tr/en. We would like to thank Prof. I. Safa Gurcan from Biostatistic Department of Ankara University Faculty of Veterinary Medicine for support in statistical analysis. Graphical abstract was prepared by using BioRender: Scientific Image and Illustration Software.
dc.identifier.doi10.1016/j.vetpar.2024.110186
dc.identifier.issn0304-4017
dc.identifier.issn1873-2550
dc.identifier.pmid38640875
dc.identifier.scopus2-s2.0-85190506270
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.vetpar.2024.110186
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24809
dc.identifier.volume328
dc.identifier.wosWOS:001306482400001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofVeterinary Parasitology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectToxocara canis; Infective stage larvae; Dog; Neutrophil; Extracellular traps; In vitro
dc.titleExtracellular traps development in canine neutrophils induced by infective stage Toxocara canis larvae
dc.typeArticle

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