Investigation of the Effects of Storage Period for Frozen Bull Semen on In Vitro Embryo Production

dc.contributor.authorAkyol, Numan
dc.contributor.authorErtem, Talha Burak
dc.contributor.authorVarisli, Omer
dc.date.accessioned2020-06-25T18:34:18Z
dc.date.available2020-06-25T18:34:18Z
dc.date.issued2019
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe aim of this study was to investigate some spermatological parameters of frozen Brown Swiss bull semen 32 years ago by flow cytometry and to determine how storage time in liquid nitrogen affects in vitro embryo production ratios. For this purpose, early necrotic, necrotic, viable and apoptotic spermatozoa concentrations were analyzed using the Flow Cytometry and then the in vitro fertilization abilities of these sperms were investigated. AnnexinV/PI-FITC (R) was used to determine the apoptotic changes with flow cytometric analysis. Oocytes were obtained from slaughtered cows in a local abattoir. Brown Swiss semen, frozen in the last two years, were used for the control group. Early necrotic spermatozoa levels in semen frozen 32 years ago were lower but necrotic spermatozoa levels were higher than in the control group, but the opposite result was encountered in the control group (P<0.01) according to flow cytometry findings. The cleavage ratio in vintage spermatozoa was found to be lower than in the control group (P<0.0z), the blastocyst ratio was also lower than in the control group (P<0.01). As a result, it was observed that some spermatological parameters can be changed negatively and it can be said that a long storage period may lower the fertility capabilities of Brown Swiss bull semen.en_US
dc.description.sponsorshipKirikkale University Scientific Research Projects Coordination UnitKirikkale University [2017/016]en_US
dc.description.sponsorshipThis research has been funded by Kirikkale University Scientific Research Projects Coordination Unit. Project Number: 2017/016.en_US
dc.identifier.citationAkyol N., Ertem T. B., Varışlı Ö. (2019). Investigation of the Effects of Storage Period for Frozen Bull Semen on In Vitro Embryo Production. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25(2), 257 - 262.en_US
dc.identifier.doi10.9775/kvfd.2018.20839
dc.identifier.endpage262en_US
dc.identifier.issn1300-6045
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85061002526
dc.identifier.scopusqualityQ3
dc.identifier.startpage257en_US
dc.identifier.trdizinid339615
dc.identifier.urihttps://doi.org/10.9775/kvfd.2018.20839
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7860
dc.identifier.volume25en_US
dc.identifier.wosWOS:000455022100017
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBrown Swiss bullen_US
dc.subjectSpermatozoaen_US
dc.subjectLong term storageen_US
dc.subjectFlow cytometryen_US
dc.subjectIn vitro fertilizationen_US
dc.titleInvestigation of the Effects of Storage Period for Frozen Bull Semen on In Vitro Embryo Productionen_US
dc.typeArticle

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