Molecular and functional analysis of a novel recombinant clone of rat (Rattus norvegicus) CD40 ligand (CD40L) gene

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dc.contributor.authorEsendağlı, Güneş
dc.contributor.authorGünel-Özcan, Ayşen
dc.contributor.authorCanpınar, Hande
dc.contributor.authorGüç, Dicle
dc.date.accessioned2020-06-25T17:48:58Z
dc.date.available2020-06-25T17:48:58Z
dc.date.issued2009
dc.departmentKırıkkale Üniversitesi
dc.descriptionEsendagli, Gunes/0000-0003-4865-2377;
dc.description.abstractGenetic material obtained from various individuals may contain certain polymorphisms which may conflict with the predetermined DNA sequence and consequently, may modulate the function of gene products. In this study, coding sequence of rat CD40 ligand (CD40L, CD154) was obtained from activated splenocytes, amplified, and cloned into a eukaryotic expression vector by using directional cloning method. Sequence of the recombinant rat CD40L DNA, pCD40L-IRES2-EGFP (pCD40L), was compared with the previously reported rat CD40L cDNA sequences and a 99% identity was found. Differing nucleotides were on the positions; 122-T/C, 341-G/A, 476G/A, 762-T/A. Further alignment analysis showed that pCD40L was collectively carrying the nucleotides each previously reported by different groups. The sequence was submitted to NCBI GenBank and nucleotide database accession number EF066490 was obtained. Following transfection of the construct into NIH/3T3 cell line, novel CD40L clone was functionally expressed de novo, increasing the expression of CD80 and CD86 costimulatory molecules and augmenting the proliferation rate of effector splenocytes in immune reactions ex vivo. Based on these data, here we report a novel recombinant clone of the rat CD40L gene which may represent a potential polymorphic variant.en_US
dc.description.sponsorshipEczacibasi Scientific Research and Award Fund; Hacettepe UniversityHacettepe University [05DO3104001]en_US
dc.description.sponsorshipThis study was supported by Eczacibasi Scientific Research and Award Fund, and Hacettepe University Scientific Research Unit (project no. 05DO3104001).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1007/s11033-007-9154-7
dc.identifier.endpage89en_US
dc.identifier.issn0301-4851
dc.identifier.issue1en_US
dc.identifier.pmid17922253
dc.identifier.scopus2-s2.0-56649106484
dc.identifier.scopusqualityQ2
dc.identifier.startpage83en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-007-9154-7
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4603
dc.identifier.volume36en_US
dc.identifier.wosWOS:000262088100011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCD40Len_US
dc.subjectImmune responseen_US
dc.subjectMolecular cloningen_US
dc.subjectRattus norvegicusen_US
dc.titleMolecular and functional analysis of a novel recombinant clone of rat (Rattus norvegicus) CD40 ligand (CD40L) geneen_US
dc.typeArticle

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