Congo Red attached monosize poly(HEMA-co-MMA) microspheres for use in reversible enzyme immobilisation

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dc.contributor.authorYavuz, H.
dc.contributor.authorBayramoğlu, G.
dc.contributor.authorKaçar, Y.
dc.contributor.authorDenizli, A.
dc.contributor.authorArica, M.Y.
dc.date.accessioned2020-06-25T17:35:07Z
dc.date.available2020-06-25T17:35:07Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.description.abstractMonosize and non-porous poly(2-hydroxyethylmethacrylate-co-methylmethacrylate) (poly(HEMA-co-MMA)), microspheres were prepared by dispersion polymerisation of HEMA and MMA in an ethanol-water medium in the presence of an initiator (alpha,alpha'-azobisisobutyronitrile, AIBN). An affinity dye, i.e. Congo Red (CR) was attached covalently and then Fe3+ ions were incorporated. The poly(HEMA-co-MMA)-CR attached and poly(HEMA-co-MMA)-CR-Fe3+ incorporated microspheres were used in the immobilisation of glucose oxidase (GOD) via adsorption. The adsorption capacities of these microspheres were determined by varying the concentration of GOD in the adsorption medium. GOD adsorption capacities of the Fe3+ incorporated microspheres (165 mg g(-1)) was greater than that of the dye-attached microspheres (126 mg g(-1)). The non-specific adsorption of the GOD on the poly(HEMA-co-MMA) microspheres was negligible. The K values for both immobilised poly(HEMA-co-MMA)-CR-GOD (7.2) and poly(HEMA-co-MMA)-CR-Fe3+-GOD (6.8) were higher than that of the free enzyme (6.6 mM). Optimum reaction pH was 5.0 for free and 7.0 for both immobilised preparations. Optimum reaction temperature of the adsorbed enzymes was 10degreesC higher than that of the free enzyme and was significantly broader. After 10 successive uses the retained activity of the adsorbed enzyme was 93%. It was observed that enzyme could be repeatedly adsorbed and desorbed on the CR attached poly(HEMA-co-MMA) microspheres without significant loss in adsorption capacity or enzyme activity. (C) 2002 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S1369-703X(01)00146-2
dc.identifier.endpage8en_US
dc.identifier.issn1369-703X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-0036163927
dc.identifier.scopusqualityQ2
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/S1369-703X(01)00146-2
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3025
dc.identifier.volume10en_US
dc.identifier.wosWOS:000173855000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Saen_US
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectglucose oxidaseen_US
dc.subjectimmobilisationen_US
dc.subjectadsorptionen_US
dc.subjectCongo Reden_US
dc.subjectenzyme technologyen_US
dc.subjectaffinity microcarriersen_US
dc.subjectpoly(HEMA-co-MMA)en_US
dc.titleCongo Red attached monosize poly(HEMA-co-MMA) microspheres for use in reversible enzyme immobilisationen_US
dc.typeArticle

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