RADIOSENSITIVITY OF HUMAN BRONCHIAL EPITHELIAL CELLS WITH GENETIC ALTERATIONS

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dc.contributor.authorDikmen, Z. Gunnur
dc.contributor.authorDikmen, Erkan
dc.contributor.authorDogan, Pakize
dc.date.accessioned2025-01-21T16:43:52Z
dc.date.available2025-01-21T16:43:52Z
dc.date.issued2005
dc.departmentKırıkkale Üniversitesi
dc.description.abstractPurpose: To analyze the proliferation rates and the radiosensitivity of human bronchial epithelial cells (HBECs) with genetic alterations including human telomerase reverse transcriptase (hTERT), Cdk4 (cyclin dependent kinase 4), knockdown p53 and mutant K-ras overexpression. Materials and Methods: HBECs obtained from human bronchus specimens were placed into short-term culture and were serially transfected with retroviral constructs containing Cdk4 and hTERT, resulting in continuously growing immortalized normal human bronchial epithelial cell lines. These cell lines were used to produce knock-down p53, mutant K-ras and knockdown p53 plus K-ras expressing cell lines; the proliferation rates and the radiosensitivity of these cell lines were evaluated. The control cells and the Cdk4, hTERT, knockdown p53 and K-ras expressing cells were irradiated (1 Gy-10 Gy) and the fraction survival was determined 1 week later. Results: The proliferation rates of the cells expressing both knockdown p53 and K-ras were higher than those of the other cell lines and show a loss of contact inhibition in vitro. The HBECs infected with both knockdown p53 and mutant K-ras were more radioresistant compared to cells infected with only mutant K-ras or knockdown p53. Conclusion: HBECs expressing knockdown p53 in addition to mutant Kras have higher proliferation rates and additionally they lose the ability of growth arrest in response to DNA damage signals such as irradiation.
dc.description.sponsorshipSimmons Cancer Center; UT Southwestern / MD Anderson Lung SPORE [CA70907]; TUBITAK (The Scientific and Technical Research Council of Turkey); TEV (Turkish Education Foundation)
dc.description.sponsorshipWe are grateful to Dr. Ruben D. Ramirez, Dr. Melville B. Vaughan,Dr. Woodring E. Wright and Dr. Jerry W. Shay from the University of Texas Southwestern for their assistance with technical development. This research was supported by the research fund from Simmons Cancer Center, the UT Southwestern / MD Anderson Lung SPORE CA70907, TUBITAK (The Scientific and Technical Research Council of Turkey) and TEV (Turkish Education Foundation).
dc.identifier.endpage87
dc.identifier.issn2147-2092
dc.identifier.issue2
dc.identifier.startpage84
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25352
dc.identifier.volume16
dc.identifier.wosWOS:000217297200006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherGazi Univ, Fac Med
dc.relation.ispartofGazi Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectHuman Bronchial Epithelial Cells; Cdk4; Htert; P53; K-Ras; Irradiation
dc.titleRADIOSENSITIVITY OF HUMAN BRONCHIAL EPITHELIAL CELLS WITH GENETIC ALTERATIONS
dc.typeArticle

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