Immobilization of glucoamylase onto spacer-arm attached magnetic poly(methylmethacrylate) microspheres: characterization and application to a continuous flow reactor

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dc.contributor.authorArica, MY
dc.contributor.authorYavuz, H
dc.contributor.authorPatir, S
dc.contributor.authorDenizli, A
dc.date.accessioned2020-06-25T17:34:40Z
dc.date.available2020-06-25T17:34:40Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractMagnetic poly(methylmethacrylate) microspheres (MPMMA) were prepared by the solvent evaporation method and a 6-carbon spacer-arm (i.e. hexamethylene diamine, HMDA) was covalently attached by the reaction of carbonyl groups of poly(methylmethacrylate). Glucoamylase was then covalently immobilized through the spacer-arm of the MPMMA microspheres by using either carbodiimide (CDI) or cyanogen bromide (CNBr) as a coupling agent. The activity yield of the immobilized glucoamylase was 57% for CDI coupling and 73% for CNBr coupling. Kinetic parameters were determined for both immobilized glucoamylase preparations as well as for the free enzyme. The K-m values for immobilized glucoamylases CDI coupling (12.5 g l(-1) dextrin) and CNBr coupling (9.3 g l(-1) dextrin) were higher than that of the free enzyme (2.1 g l(-1) dextrin) whereas V-max values were smaller for the immobilized glucoamylase preparations. The optimum operational temperature was 5 degreesC higher for both immobilized preparations than that of the free enzyme. Operational, thermal and storage stabilities were found to be increased with immobilization. (C) 2000 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S1381-1177(00)00223-X
dc.identifier.endpage138en_US
dc.identifier.issn1381-1177
dc.identifier.issue2-3en_US
dc.identifier.scopus2-s2.0-0034642369
dc.identifier.scopusqualityN/A
dc.identifier.startpage127en_US
dc.identifier.urihttps://doi.org/10.1016/S1381-1177(00)00223-X
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2833
dc.identifier.volume11en_US
dc.identifier.wosWOS:000165463000007
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal Of Molecular Catalysis B-Enzymatic
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectglucoamylaseen_US
dc.subjectenzyme immobilizationen_US
dc.subjectcovalent bondingen_US
dc.subjectspacer-armen_US
dc.subjectenzyme reactoren_US
dc.subjectmagnetic microspheresen_US
dc.subjectpoly(methylmethacrylate)en_US
dc.titleImmobilization of glucoamylase onto spacer-arm attached magnetic poly(methylmethacrylate) microspheres: characterization and application to a continuous flow reactoren_US
dc.typeArticle

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