Evaluation of the effect of enriched hydrogen saline solution on distant organ (lung) damage in skeletal muscle ischemia reperfusion in rats

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dc.authoridArslan, Mustafa/0000-0003-4882-5063
dc.contributor.authorOzer, Abdullah
dc.contributor.authorErel, Selin
dc.contributor.authorKucuk, Aysegul
dc.contributor.authorDemirtas, Hueseyin
dc.contributor.authorSezen, Saban Cem
dc.contributor.authorBoyunaga, Hakan
dc.contributor.authorOktar, Gursel Levent
dc.date.accessioned2025-01-21T16:40:44Z
dc.date.available2025-01-21T16:40:44Z
dc.date.issued2024
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIntroduction Ischemia-reperfusion (IR) injury is a major concern that frequently occurs during vascular surgeries. Hydrogen-rich saline (HRS) solution exhibits antioxidant and anti-inflammatory properties. This study aimed to examine the effects of HRS applied before ischemia in the lungs of rats using a lower extremity IR model. Material and Methods: After approval was obtained from the ethics committee, 18 male Wistar albino rats weighing 250-280 g were randomly divided into three groups: control (C), IR and IR-HRS. In the IR and IR-HRS groups, an atraumatic microvascular clamp was used to clamp the infrarenal abdominal aorta, and skeletal muscle ischemia was induced. After 120 min, the clamp was removed, and reperfusion was achieved for 120 min. In the IR-HRS group, HRS was administered intraperitoneally 30 min before the procedure. Lung tissue samples were examined under a light microscope and stained with hematoxylin-eosin (H&E). Malondialdehyde (MDA) levels, total sulfhydryl (SH) levels, and histopathological parameters were evaluated in the tissue samples. Results: MDA and total SH levels were significantly higher in the IR group than in the control group (p < 0.0001 and p = 0.001, respectively). MDA and total SH levels were significantly lower in the IR-HRS group than in the IR group (p < 0.0001 and p = 0.013, respectively). A histopathological examination revealed that neutrophil infiltration/aggregation, alveolar wall thickness, and total lung injury score were significantly higher in the IR group than in the control group (p < 0.0001, p = 0.001, and p < 0.0001, respectively). Similarly, alveolar wall thickness and total lung injury scores were significantly higher in the IR-HRS group than in the control group (p = 0.009 and p = 0.004, respectively). A statistically significant decrease was observed in neutrophil infiltration/aggregation and total lung injury scores in the IR-HRS group compared to those in the IR group (p = 0.023 and p = 0.022, respectively). Conclusion: HRS at a dose of 20 mg/kg, administered intraperitoneally 30 min before ischemia in rats, reduced lipid peroxidation and oxidative stress, while also reducing IR damage in lung histopathology. We believe that HRS administered to rats prior to IR exerts a lung-protective effect.
dc.identifier.doi10.1177/00368504241257060
dc.identifier.issn0036-8504
dc.identifier.issn2047-7163
dc.identifier.issue2
dc.identifier.pmid38807538
dc.identifier.scopus2-s2.0-85194855543
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1177/00368504241257060
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24758
dc.identifier.volume107
dc.identifier.wosWOS:001235439300001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Ltd
dc.relation.ispartofScience Progress
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectHydrogen-rich saline solution; ischemia-reperfusion; lung; biochemical analysis; histopathological analysis
dc.titleEvaluation of the effect of enriched hydrogen saline solution on distant organ (lung) damage in skeletal muscle ischemia reperfusion in rats
dc.typeArticle

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