A dye-ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin G

Basit Öğe Kaydı
dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorÖktem, H. Avni
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2020-06-25T17:43:48Z
dc.date.available2020-06-25T17:43:48Z
dc.date.issued2007
dc.description.abstractPoly(2-hydroxyethylmethaerylate) (pHEMA) membrane was prepared by photo-polymerization and a dye ligand (i.e., Reactive Green 5) was immobilised on the membrane surface (pHEMA-RG-5). Surface wettability and hydrophilicity of the dye-ligand immobilised membranes were investigated by static contact angle measurements. The chromatographic properties of the dye-ligand immobilized membrane for adsorption and purification of IgG are discussed using bare membrane as a control system. The non-specific adsorption of IgG was low (0.45 mg protein ml(-1) membrane). The attachment of dye ligand onto the membrane significantly increased the IgG adsorption (33.75 mg protein ml(-1) membrane). The maximum IgG adsorption was observed at pH 6.0. The reversible IgG adsorption on the membrane obeyed both the Freundlich and the Langmuir-Freundlich isotherm models. To test the efficiency of IgG isolation from human serum with the pHEMA-RG-5 membrane, the purity of the eluted IgG, as determined by HPLC, was 81% with a recovery of 67%. (c) 2006 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.bej.2006.11.025
dc.identifier.endpage155en_US
dc.identifier.issn1369-703X
dc.identifier.issn1873-295X
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-33947330124
dc.identifier.scopusqualityQ2
dc.identifier.startpage147en_US
dc.identifier.urihttps://doi.org10.1016/j.bej.2006.11.025
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3904
dc.identifier.volume34en_US
dc.identifier.wosWOS:000245828500007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectadsorptionen_US
dc.subjectbiomimeticsen_US
dc.subjectmicroporous membranesen_US
dc.subjectseparationen_US
dc.subjectIgGen_US
dc.subjectcontact angleen_US
dc.titleA dye-ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin Gen_US
dc.typeArticle

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