Evaluation of matrix metalloproteinase, myeloperoxidase, and oxidative damage in mesenteric ischemia-reperfusion injury

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dc.contributor.authorKocael, A.
dc.contributor.authorInal, B. B.
dc.contributor.authorGuntas, G.
dc.contributor.authorKelten, C.
dc.contributor.authorInal, H.
dc.contributor.authorTopac, H. I.
dc.contributor.authorUzun, H.
dc.date.accessioned2020-06-25T18:16:22Z
dc.date.available2020-06-25T18:16:22Z
dc.date.issued2016
dc.departmentKırıkkale Üniversitesi
dc.descriptionSalihoglu, Ziya/0000-0002-6905-2664; Uzun, Hafize/0000-0002-1347-8498
dc.description.abstractBackground: In this study, we investigated the alterations of matrix metalloproteinase (MMP) and tissue inhibitors of metalloproteinases (TIMPs), acute inflammation, and oxidative damage in the circulatory system and the intestine in response to mesenteric ischemia/reperfusion (I/R). Methods: Twenty-one rats were divided randomly into the following three groups (n = 7 in each group): a sham group (CG), an ischemic group (IG), and an I/R group (I/RG). MMP-9, TIMP-1, and myeloperoxidase (MPO) were measured using the enzyme-linked immunosorbent assay method, and lipid peroxidation (quantified as thiobarbituric acid reactive substances (TBARS) content), ischemia-modified albumin, the prooxidant-antioxidant balance (PAB), and ferric-reducing antioxidant power (FRAP) were measured spectrophotometrically. The degree of intestinal injury was evaluated according to the Chiu scoring system. Results: A significant difference between the mean serum TIMP-1 and MMP-9 levels and the alanine transaminase activity was found among the groups. Compared with the I/RG group a significant difference in the mean tissue MMP-9, MPO, and TBARS levels in addition to the PAB and FRAP was found between the CG and IG groups. The level of MMP-9 also demonstrated a strong, positive, and valid correlation with the TBA-RS levels. A significant morphological change was observed in both the IG and the I/RG groups. The degree of intestinal injury was more severe in the I/R group and was characterized by either villous denudation or villous loss. Conclusions: These results suggest that MMP-9, TIMP-1, MPO, and oxidative stress may be important in the intestinal injury development that is induced by acute mesenteric I/R in a rat model. MMP-9 overexpression may increase the extent of intestinal villous loss, particularly when MMP-9 is upregulated by the TBARS present in the intestinal injury.en_US
dc.description.sponsorshipSiemens Healthcare Diagnostic Inc., West Sacramento, California, USAen_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by Siemens Healthcare Diagnostic Inc., West Sacramento, California, USA.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1177/0960327115607946
dc.identifier.endpage860en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue8en_US
dc.identifier.pmid26429927
dc.identifier.startpage851en_US
dc.identifier.urihttps://doi.org/10.1177/0960327115607946
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6506
dc.identifier.volume35en_US
dc.identifier.wosWOS:000380943600006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMesenteric ischemiaen_US
dc.subjectreperfusionen_US
dc.subjectmatrix metalloproteinase 9en_US
dc.subjecttissue inhibitor of metalloproteinase 1en_US
dc.subjectmyeloperoxidaseen_US
dc.subjectoxidative damageen_US
dc.subjectintestinal ischemic injuryen_US
dc.titleEvaluation of matrix metalloproteinase, myeloperoxidase, and oxidative damage in mesenteric ischemia-reperfusion injuryen_US
dc.typeArticle

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