In vitro cadmium removal from human serum by Cibacron Blue F3GA-thionein-complex conjugated affinity membranes

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dc.contributor.authorDenizli, F
dc.contributor.authorDenizli, A
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:34:44Z
dc.date.available2020-06-25T17:34:44Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractA new membrane affinity biosorbent carrying thionein has been developed for selective removal of cadmium ions from human serum. Microporous poly(2-hydroxyethyl methacrylate) (pHEMA) membranes were prepared by photopolymerization of HEMA. The pseudo dye ligand Cibacron Blue F3GA (CB) was covalently immobilized on the pHEMA membranes. Then, the cysteine-rich metallopeptide thionein was conjugated onto the CB-immobilized membrane. The maximum amounts of CB immobilized and thionein conjugated on the membranes were 1.07 mu mol cm(-2) and 0.92 mu mol cm(-2), respectively. The hydrophilic pHEMA membrane had a swelling ratio of 58% (w/w) with a contact angle of 45.8 degrees. CB-immobilized and CB-immobilized-thionein-conjugated membranes were used in the Cd(II) removal studies. Cd(II) ion adsorption appeared to reach equilibrium within 30 min and to follow a typical Langmuir adsorption isotherm. The maximum capacity (q(m)) of the CB-immobilized membranes was 0.203 (mu mol Cd(II))cm(-2) membrane and increased to 1.48 (mu mol Cd(II))cm(-2) upon CB-thionein-complex conjugation. The pHEMA membranes retained their cadmium adsorption capacity even after 10 cycles of repeated use. (C) 2000 Society of Chemical Industry.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/(SICI)1097-0126(200003)49:3<302
dc.identifier.endpage308en_US
dc.identifier.issn0959-8103
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0034156370
dc.identifier.scopusqualityQ1
dc.identifier.startpage302en_US
dc.identifier.urihttps://doi.org/10.1002/(SICI)1097-0126(200003)49:3<302
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2866
dc.identifier.volume49en_US
dc.identifier.wosWOS:000085651300011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Ltden_US
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpoly(2-hydroxyethyl methacrylate)en_US
dc.subjectaffinity membraneen_US
dc.subjectCibacron Blue F3GAen_US
dc.subjectpeptideen_US
dc.subjectthioneinen_US
dc.subjectcomplex conjugationen_US
dc.subjectcadmiumen_US
dc.subjecthuman serumen_US
dc.titleIn vitro cadmium removal from human serum by Cibacron Blue F3GA-thionein-complex conjugated affinity membranesen_US
dc.typeArticle

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