Preparation and application of spacer-arm-attached poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) films for urease immobilisation

Basit Öğe Kaydı
dc.contributor.authorBayramoglu, G
dc.contributor.authorAltinok, H
dc.contributor.authorBulut, A
dc.contributor.authorDenizli, A
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:35:42Z
dc.date.available2020-06-25T17:35:42Z
dc.date.issued2003
dc.departmentKırıkkale Üniversitesi
dc.description.abstractImmobilisation of urease onto plain and six-carbon spacer-arm (i.e. 1,6-diaminohexane) attached poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) [poly (HEMA-GMA)] films was studied. The epoxy groups containing poly(HEMA-GMA-1-3) films were prepared by UV-initiated photopolymerisation of 2-hydroxyethyl methacrylate (HEMA) and glycidyl methacrylate (GMA). The spacer arm (i.e. 1,6-diaminohexane) was attached through the epoxy groups of the films. Urease was then covalently immobilised either on the plain or on the spacer-arm-attached poly(HEMA-GMA-2) films using Glutaric dialdehyde as a coupling agent. Incorporation of the spacer-arm resulted in an increase in the apparent activity of the immobilised urease with respect to the enzyme immobilised on the plain films. The urease immobilisation capacity of the films was increased as the GMA ratio increased in the film structure. The activity yield of the immobilised urease on the spacer-arm-attached poly(HEMA-GMA-2) films was 53%, and 36% for the enzyme immobilised on the plain poly(HEMA-GMA-2) films. Both immobilised urease preparations have resistance to temperature inactivation when compared to that of the free form. The temperature profiles were broader for both immobilised preparations than that of the free enzyme. Kinetic parameters were determined for immobilised urease preparations as well as for the free enzyme. The values of the Michaelis constants K(m) for all the immobilised urease preparations were significantly larger, indicating decreased affinity by the enzyme for its substrate, whereas V(max) values were smaller for both immobilised urease preparations. In an 80-h continuous operation with spacer-arm-attached poly(HEMA-GMA-2) films at 35 degreesC, only 7% of immobilised urease activity was lost. The operational inactivation rate constant (k(opi)) of the immobilised urease was 1.51 x 10(-5) min(-1). (C) 2003 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S1381-5148(03)00050-6
dc.identifier.endpage121en_US
dc.identifier.issn1381-5148
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-0141641130
dc.identifier.scopusqualityQ1
dc.identifier.startpage111en_US
dc.identifier.urihttps://doi.org/10.1016/S1381-5148(03)00050-6
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3187
dc.identifier.volume56en_US
dc.identifier.wosWOS:000185837700003
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofReactive & Functional Polymers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpolymer filmen_US
dc.subjectimmobilisationen_US
dc.subjectureaseen_US
dc.subjecturea degradationen_US
dc.subjectenzyme tank reactoren_US
dc.titlePreparation and application of spacer-arm-attached poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) films for urease immobilisationen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Preparation and application of spacer-arm-attached poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) films for urease immobilisation.pdf
Boyut:
188.11 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu