Affinity dye-ligand poly(hydroxyethyl methacrylate)/chitosan composite membrane for adsorption lysozyme and kinetic properties

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dc.contributor.authorBayramoglu, G
dc.contributor.authorYilmaz, M
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:35:39Z
dc.date.available2020-06-25T17:35:39Z
dc.date.issued2003
dc.departmentKırıkkale Üniversitesi
dc.description.abstractA composite membrane from 2-hydroxyethyl methacrylate (HEMA) and poly(hydroxyethyl methacrylate)/chitosan (pHEMA/chitosan) was synthesized via UV initiated photo-polymerization in the presence of an initiator alpha,alpha'-azoisobutyronitrile (AIBN). Procion Brown MX 5BR was then covalently immobilized onto composite membrane as a dye-ligand. The binding characteristics of a model protein (i.e. lysozyme) to the dye-ligand immobilized affinity membrane have been investigated from aqueous solution using the plain composite membrane as a control system. The experimental data was analyzed using two adsorption kinetic models, the pseudo-first-order and the pseudo-second-order, to determine the best-fit equation for the adsorption of lysozyme onto affinity composite membrane. The second-order equation for the adsorption of lysozyme on the dye-ligand membrane systems is the most appropriate equation to predict the adsorption capacity for the affinity membrane. The reversible lysozyme adsorption on the affinity membrane obeyed the Freundlich isotherm. The lysozyme adsorption capacity of the plain membrane and the dye-ligand affinity membrane were 8.3 and 121.5 mg ml(-1), respectively. (C) 2002 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S1369-703X(02)00098-0
dc.identifier.endpage42en_US
dc.identifier.issn1369-703X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-0037231070
dc.identifier.scopusqualityQ2
dc.identifier.startpage35en_US
dc.identifier.urihttps://doi.org/10.1016/S1369-703X(02)00098-0
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3176
dc.identifier.volume13en_US
dc.identifier.wosWOS:000180915500005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Saen_US
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectadsorptionen_US
dc.subjectaffinity chromatographyen_US
dc.subjectcomposite membraneen_US
dc.subjectdye-liganden_US
dc.subjectkinetic parametersen_US
dc.subjectlysozymeen_US
dc.subjectproteinen_US
dc.titleAffinity dye-ligand poly(hydroxyethyl methacrylate)/chitosan composite membrane for adsorption lysozyme and kinetic propertiesen_US
dc.typeArticle

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