Pektin, poligalakturonik asit ve liyofilize pektinaz enziminin yapısal analizi
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Dosyalar
Tarih
2004
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Pektik enzimler, yüksek moleküler ağırlıklı pektik asitlerin indirgeyici olmayan uçlarına atak yaparak monogalakturonatları oluşturan enzimlerdir. Bu enzimler Saprofltik fungus, bakteri ve bazı mayalarca oluşturulmaktadırlar. Meyve ve sebze teknolojilerinde kullanılmaktadır. Bu çalışmada pektin, poligalakturonik asit ve liyofilize bakteriyel pektinazın kristal yapıları, taramalı elektron mikroskobunda (SEM) görüntülenmiştir. SEM'deki görüntülerinde homojen kristal yapı görülmektedir. Pektin poligalakturonik asit ve bakteriyel pektinaz, elektron ışınlarını geçilmeyecek kadar kompakt ve sıkı paketlenmiştir. Ayrıca substrat moleküllerinin kristallerinin pektinaz enziminden daha küçük boyutta olduğu tespit edilmiştir. Pektinazın enzimatik aktivitesini Ca{} ve Na gibi katyonların sitimüle ettiği bilinmektedir. Çalışmanın ikinci aşamasında pektinaz enziminin kristal yapısında bulunabilecek elementlerin analizi gerçekleştirilmiştir.
Pectic substances are pectinic acid, pectin, pectic acid and their salts. Pectin is a polysaccaride found in plants like fruits and vegetables etc in high level. Substances which have no methyl group are pectic acid and polygalacturonic acid. Pectic substances are heteropolysaccarides with 30,000-300,000 molecular weight.Pectic enzymes are known as enzyme destroying chain. Pectic enzymes produce monogalacturonates by attaking the nonreducing end of the high molecular weight pectic acids. These are produced by saprophytic fungi, bacteria and some yeasts. They are used in fruit and vegetable technologies. In this research, crystal structures of pectin, polygalacturonic acid and lyophilysed bacterial pectinase samples were studied by scanning elektron microskop. Homogenous crystal structure was observed from the images at SEM. Pectin, polygalacturonic acid and pectinase enzyme was packed so compact and tightly that no transiion of beam was observed. Pectin crystals have bigger size than polygalacturonic acid crystals. The crystals of substrat molecules was determined to be smaller than pectinase enzymes. Ca and Na cations are known to stimulate enzymatic activity In second step of study, the elements which are thought to be present in the crystal structure of pectinase were analysed. Analysis results showed that Na, Zn and Ca elements were found at concentrations of 60 %, 29.296 % and 6.555 %, respectively.
Pectic substances are pectinic acid, pectin, pectic acid and their salts. Pectin is a polysaccaride found in plants like fruits and vegetables etc in high level. Substances which have no methyl group are pectic acid and polygalacturonic acid. Pectic substances are heteropolysaccarides with 30,000-300,000 molecular weight.Pectic enzymes are known as enzyme destroying chain. Pectic enzymes produce monogalacturonates by attaking the nonreducing end of the high molecular weight pectic acids. These are produced by saprophytic fungi, bacteria and some yeasts. They are used in fruit and vegetable technologies. In this research, crystal structures of pectin, polygalacturonic acid and lyophilysed bacterial pectinase samples were studied by scanning elektron microskop. Homogenous crystal structure was observed from the images at SEM. Pectin, polygalacturonic acid and pectinase enzyme was packed so compact and tightly that no transiion of beam was observed. Pectin crystals have bigger size than polygalacturonic acid crystals. The crystals of substrat molecules was determined to be smaller than pectinase enzymes. Ca and Na cations are known to stimulate enzymatic activity In second step of study, the elements which are thought to be present in the crystal structure of pectinase were analysed. Analysis results showed that Na, Zn and Ca elements were found at concentrations of 60 %, 29.296 % and 6.555 %, respectively.
Açıklama
Anahtar Kelimeler
Kaynak
İTÜ Dergisi Seri C: Fen Bilimleri
WoS Q Değeri
Scopus Q Değeri
Cilt
2
Sayı
1
Künye
Maraş, M., Çavuşoğlu, K., Aksöz, E., Kırındı, T. (2004). Pektin, poligalakturonik asit ve liyofilize pektinaz enziminin yapısal analizi. İTÜ Dergisi Seri C: Fen Bilimleri, 2(1), 3 - 10.
