In vitro sensitivity of mouse esophagus to agonists in different pH medium values

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dc.contributor.authorSoyer, Tutku
dc.contributor.authorKeskil, Zuhal Aktuna
dc.contributor.authorSomuncu, Salih
dc.contributor.authorAydos, Tolga Reşat
dc.contributor.authorKorkut, Oğuzhan
dc.contributor.authorKanmaz, Turan
dc.contributor.authorÇakmak, Murat
dc.date.accessioned2020-06-25T17:43:35Z
dc.date.available2020-06-25T17:43:35Z
dc.date.issued2007
dc.description40th Annual Meeting of the Pacific-Association-of-Pediatric-Surgeons -- APR 15-19, 2007 -- Queenstown, NEW ZEALAND
dc.descriptionAydos, Tolga Resat/0000-0002-1832-9336; Soyer, Tutku/0000-0003-1505-6042
dc.description.abstractAim: The aim of this study is to determine the in vitro sensitivity of mouse esophagus to contracting and relaxing agonists in different pH medium values. Materials and Methods: Forty-eight Swiss albino mice (30-40 g) of both sexes were anesthetized with tiopental sodium (30 mg/kg). After exsanguinations from abdominal artery, esophagi were removed and suspended under 0.6 g of resting tension in a tissue bath containing 10 mL of Krebs solution at 37 C. The experiments were performed in different pH mediums 7.4, 6.4, 4, and 2. Carbachol and acetylcholine were used as contractile agonists, and noradrenalin and isoproterenol to evaluate relaxation responses. Data concerning similar concentrations of contractile agonists obtained from different pH mediums were analyzed using Kruskal-Wallis nonparametric analysis of variance and post hoc Dunn test. Relaxation responses were compared with Student t test. A P value less than .05 was considered significant. The study was approved by Local Ethical Committee of Kmkkale University. Results: Carbachol and acetylcholine caused concentration-dependent contractility in pH 7.4, 6.4, and 4, but contractile responses were inhibited in pH 2. In carbachol and acetylcholine experiments, there was a significant decrease in contractile responses to all concentrations in conjunction with a decreased in pH value. Relaxation responses in pH 2 and 4 could not be obtained because precontraction of tissues was not possible. Noradrenalin and isoproterenol produced concentration-dependent relaxations in pH 7.4 and 6.4. Although noradrenalin responses showed no significant difference according to pH, isoproterenol caused better relaxations in pH 6.4 (between 10(-8) and 10(-6) mol/L) when compared to pH 7.4 studies. Conclusion: The mouse esophagus has impaired contractile responses to carbachol and acetylcholine in decreased pH values. Contraction responses did not occur in pH medium of 2. In contrast, esophagus segments showed better relaxations in lower pH values with isoproterenol. (c) 2007 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipPacific Assoc Pediat Surgen_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.jpedsurg.2007.08.006
dc.identifier.endpage1992en_US
dc.identifier.issn0022-3468
dc.identifier.issue12en_US
dc.identifier.pmid18082693
dc.identifier.scopus2-s2.0-36849061088
dc.identifier.scopusqualityQ1
dc.identifier.startpage1988en_US
dc.identifier.urihttps://doi.org10.1016/j.jpedsurg.2007.08.006
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3810
dc.identifier.volume42en_US
dc.identifier.wosWOS:000252229900003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherW B Saunders Co-Elsevier Incen_US
dc.relation.ispartofJournal Of Pediatric Surgery
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectesophagusen_US
dc.subjectagonisten_US
dc.subjectpHen_US
dc.titleIn vitro sensitivity of mouse esophagus to agonists in different pH medium valuesen_US
dc.typeConference Object

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