In vivo imaging/detection of MRSA bacterial infections in mice using fluorescence labelled polymeric nanoparticles carrying vancomycin as the targeting agent

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dc.contributor.authorDizaji, Araz Norouz
dc.contributor.authorDing, Dan
dc.contributor.authorKutsal, Tulin
dc.contributor.authorTurk, Mustafa
dc.contributor.authorKong, Deling
dc.contributor.authorPiskin, Erhan
dc.date.accessioned2021-01-14T18:11:11Z
dc.date.available2021-01-14T18:11:11Z
dc.date.issued2020
dc.departmentKKÜ
dc.descriptionDizaji, Araz Norouz/0000-0001-6720-2115
dc.description.abstractThis study aims to develop fluorescence labelled polymeric nanoparticle (NP) carrying vancomycin as the targeting agent for in vivo imaging of Methicillin-resistant Staphylococcus aureus bacterial infections in animal models. Maleimide functionalized 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000] as the main was carrier matrix to prepare the NPs. A fluorescence probe, namely, poly[9,9 '-bis (6 ''-N,N,N-trimethylammonium) hexyl) fluorene-co-alt-4,7-(2,1,3-benzothiadiazole) dibromide] was encapsulated within these NPs by ultrasonication successfully. UV-Vis spectro- photometry of the NPs showed the characteristic shifting on the peak of conjugated polymers indicating successful packaging of this compound with lipid bilayers in nanoscales. Zeta-sizer and TEM analysis showed that the prepared NPs have a diameter of 80-100 nm in a narrow size distribution. Thiolated vancomycin was synthesized and attached to the NPs as the targeting agent. FTIR and MALDI-TOF spectroscopy analysis confirmed the immobilization. The specific targeting properties of the vancomycin conjugated NPs to the target bacteria were first confirmed in in vitro bacterial cultures in which Escherichia coli was the non-target bacteria - using confocal microscopy and TEM. Imaging of bacterial infections in vivo was investigated in mice model using a non-invasive live animal fluorescence imaging technique. The results confirmed that bacterial infections can be detected using these novel polymeric NPs carrying fluorescence probes for imaging and vancomycin as the targeting agent - in vivo successfully.en_US
dc.description.sponsorshipTurkish Scientific and Technological CouncilTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [1130864]en_US
dc.description.sponsorshipThis study was conducted in the frame of a EU project between Europe and China - the shortly called 'ABREM', 'FP7-PEOPLE-2009-IRSES' and also supported by Turkish Scientific and Technological Council (Project Number: 1130864).en_US
dc.identifier.citationBu makale açık erişimli değildir.en_US
dc.identifier.doi10.1080/09205063.2019.1692631
dc.identifier.endpage309en_US
dc.identifier.issn0920-5063
dc.identifier.issn1568-5624
dc.identifier.issue3en_US
dc.identifier.pmid31762403
dc.identifier.scopus2-s2.0-85075535062
dc.identifier.scopusqualityQ1
dc.identifier.startpage293en_US
dc.identifier.urihttps://doi.org/10.1080/09205063.2019.1692631
dc.identifier.urihttps://hdl.handle.net/20.500.12587/12908
dc.identifier.volume31en_US
dc.identifier.wosWOS:000498377700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofJOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFluorescence probesen_US
dc.subjectdyesen_US
dc.subjectin vivo detection of bacterial infectionsen_US
dc.subjectMethicillin-resistant Staphylococcus aureusen_US
dc.subjectpolymeric nanoparticlesen_US
dc.subjecttargetingen_US
dc.subjectvancomycinen_US
dc.titleIn vivo imaging/detection of MRSA bacterial infections in mice using fluorescence labelled polymeric nanoparticles carrying vancomycin as the targeting agenten_US
dc.typeArticle

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