Staining characteristics of p16INK4a: Is there a correlation with lesion grade or high-risk human papilloma virus positivity?

Basit Öğe Kaydı
dc.contributor.authorÖzgül, Nejat
dc.contributor.authorÇil, Aylin P.
dc.contributor.authorBozdayi, Gülendam
dc.contributor.authorUsubutun, Alp
dc.contributor.authorBulbul, Dilek
dc.contributor.authorRota, Seyyar
dc.contributor.authorHaberal, Ali
dc.date.accessioned2020-06-25T17:44:18Z
dc.date.available2020-06-25T17:44:18Z
dc.date.issued2008
dc.departmentKırıkkale Üniversitesi
dc.descriptionUsubutun, Alp/0000-0001-9572-7875;
dc.description.abstractAim: The aims of this study were to evaluate the efficiency of p16INK4a in showing cervical lesions and to determine any relationship between lesion grade and high-risk human papilloma virus (HR-HPV) infection and p16INK4a staining characteristics. Methods: Immunohistochemical analysis of p16INK4a was performed on 13 low-grade squamous intraepithelial lesions (LSIL), 22 high-grade squamous intraepithelial lesions (HSIL), 23 squamous cell carcinoma (SCC) and 25 normal tissue samples. The distribution, staining pattern and intensity of p16INK4a expression were assessed and correlated with HR-HPV positivity determined by real-time polymerase chain reaction. Results: All HSIL and SCC cases, but only 46.2% of LSIL cases, were positive for p16INK4a. Although positive staining of p16INK4a in showing HR-HPV-positive lesions was statistically significant (P = 0.000), we could not find a significant correlation for distribution (P = 0.319), staining pattern (P = 0.057) or intensity (P = 0.057) of p16INK4a in showing HR-HPV in cervical epithelium. These parameters were correlated only with the increasing grade of the lesion (P = 0.000). Conclusions: p16INK4a is a highly sensitive marker of cervical intraepithelial neoplasia and cervical cancer. There is a good correlation between p16INK4a expression and cervical lesion grade and HR-HPV positivity. The distribution, staining pattern and intensity of this marker are significantly correlated with the increasing grade of cervical lesions, suggesting that diffuse distribution, full thickness staining pattern and strong intensity of this marker are highly supportive of HSIL and cervical cancer, while focal distribution, scattered/basal staining pattern and weak intensity suggest LSIL. However, these parameters are not correlated with HR-HPV status in cervical lesions.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1111/j.1447-0756.2008.00795.x
dc.identifier.endpage871en_US
dc.identifier.issn1341-8076
dc.identifier.issue5en_US
dc.identifier.pmid18958930
dc.identifier.scopus2-s2.0-52049089689
dc.identifier.scopusqualityQ2
dc.identifier.startpage865en_US
dc.identifier.urihttps://doi.org/10.1111/j.1447-0756.2008.00795.x
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4073
dc.identifier.volume34en_US
dc.identifier.wosWOS:000259270800015
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofJournal Of Obstetrics And Gynaecology Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcervical canceren_US
dc.subjectcervical intraepithelial neoplasiaen_US
dc.subjecthigh-grade squamous intraepithelial lesionen_US
dc.subjectimmunohistochemistryen_US
dc.subjectlow-grade squamous intraepithelial lesionen_US
dc.subjectp16en_US
dc.titleStaining characteristics of p16INK4a: Is there a correlation with lesion grade or high-risk human papilloma virus positivity?en_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Staining characteristics of p16INK4a Is there a correlation with lesion grade or high-risk human papilloma virus positivity.pdf
Boyut:
139.98 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu