Effects of Ganoderma lucidum Polysaccharides on Different Pathways Involved in the Development of Spinal Cord Ischemia Reperfusion Injury: Biochemical, Histopathologic, and Ultrastructural Analysis in a Rat Model

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dc.authoridKahveci, Fatih Ozan/0000-0002-6158-501X
dc.authoridGurer, Bora/0000-0003-1500-6184
dc.contributor.authorKahveci, Ramazan
dc.contributor.authorKahveci, Fatih Ozan
dc.contributor.authorGokce, Emre Cemal
dc.contributor.authorGokce, Aysun
dc.contributor.authorKisa, Ucler
dc.contributor.authorSargon, Mustafa Fevzi
dc.contributor.authorFesli, Ramazan
dc.date.accessioned2025-01-21T16:38:17Z
dc.date.available2025-01-21T16:38:17Z
dc.date.issued2021
dc.departmentKırıkkale Üniversitesi
dc.description.abstractOBJECTIVE: Inflammation and oxidative stress are 2 important factors in the emergence of paraplegia associated with spinal cord ischemia-reperfusion injury (SCIRI) after thoracoabdominal aortic surgery. Here it is aimed to investigate the effects of Ganoderma lucidum polysaccharide (GLPS) on SCIRI. METHODS: Rats were randomly selected into 4 groups of 8 animals each: sham, ischemia, methylprednisolone, and GLPS. To research the impacts of various pathways that are efficacious in formation of SCIRI, tumor necrosis factor alpha, interleukin 1 beta, nitric oxide, superoxide dismutase levels, and catalase, glutathione peroxidase activities, malondialdehyde levels, and caspase-3 activity were measured in tissues taken from the spinal cord of rats in all groups killed 24 hours after ischemia reperfusion injury. The Basso, Beattie, and Bresnahan locomotor scale and inclined plane test were used for neurologic assessment before and after SCIRI. In addition, histologic and ultrastructural analyses of tissue samples in all groups were performed. RESULTS: SCIRI also caused marked increase in tissue tumor necrosis factor alpha, interleukin 1 beta, nitric oxide, malondialdehyde levels, and caspase-3 activity, because of inflammation, increased free radical generation, lipid peroxidation, and apoptosis, respectively. On the other hand, SCIRI caused significant reduction in tissue superoxide dismutase, glutathione peroxidase, and catalase activities. Pretreatment with GLPS likewise diminished the level of the spinal cord edema, inflammation, and tissue injury shown by pathologic and ultrastructural examination. Pretreatment with GLPS reversed all these biochemical changes and improved the altered neurologic status. CONCLUSIONS: These outcomes propose that pretreatment with GLPS prevents progression of SCIRI by alleviating inflammation, oxidation, and apoptosis.c
dc.identifier.doi10.1016/j.wneu.2021.02.129
dc.identifier.endpageE297
dc.identifier.issn1878-8750
dc.identifier.issn1878-8769
dc.identifier.pmid33689849
dc.identifier.scopus2-s2.0-85103581542
dc.identifier.scopusqualityQ1
dc.identifier.startpageE287
dc.identifier.urihttps://doi.org/10.1016/j.wneu.2021.02.129
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24626
dc.identifier.volume150
dc.identifier.wosWOS:000657837500030
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofWorld Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectGanoderma lucidum polysaccharides; Ischemia/reperfusion; Neuroprotection; Spinal cord
dc.titleEffects of Ganoderma lucidum Polysaccharides on Different Pathways Involved in the Development of Spinal Cord Ischemia Reperfusion Injury: Biochemical, Histopathologic, and Ultrastructural Analysis in a Rat Model
dc.typeArticle

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