Immobilization of laccase on polyacrylamide and polyacrylamide – κ – carragennan-based semiinterpenetrating polymer networks

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dc.contributor.authorGokgoz, M.
dc.contributor.authorAltinok, H.
dc.date.accessioned2020-06-25T17:52:35Z
dc.date.available2020-06-25T17:52:35Z
dc.date.issued2012
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, laccase enzyme (L) from Agaricus bisporus was immobilized by entrapment into polyacrylamide (PAAm) and semi-interpenetrating polymer networks (semi-IPNs) prepared with either polyacrylamide/kappa-carragennan (0.05g) [PAAm/kappa-car (0.05)] or polyacrylamide/kappa-carragennan (0.1 g) [PAAm/kappa-car (0.1)]. The optimum pH was 6.0 for free L, 8.0 for PAAm-L, 8.5 for PAAm/kappa-car (0.05)-L, and 9.0 for PAAm/kappa-car (0.1)-L. The optimum temperature was determined as 45 degrees C for free L and 60 degrees C for all immobilized laccases. After 27 days of storage at 4 degrees C, free enzyme lost its initial activity whereas immobilized enzymes retained 56 % (-)80 % of their initial activities. The immobilized samples were used repeatedly 35 times by retaining 28 %-58 % of their initial activity. K-m(app) values were calculated as 0.088, 0.139, 0.133, and 0.131 mM and Vmax values were found to be 2.83 x 10(-3), 4.51 x 10(-3), 4.76 x 10(-3), and 4.97 x 10(-3) mM min(-1) for free L and PAAm-L, PAAm/kappa-car (0.05)-L, and PAAm/kappa-car (0.1)-L, respectively.en_US
dc.description.sponsorshipKirikkale University, Research Grant BAPKirikkale Universityen_US
dc.description.sponsorshipThis research was supported by Kirikkale University, Research Grant BAP.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.3109/10731199.2012.658469
dc.identifier.endpage330en_US
dc.identifier.issn1073-1199
dc.identifier.issn1532-4184
dc.identifier.issue5en_US
dc.identifier.pmid22594636
dc.identifier.scopus2-s2.0-84866982821
dc.identifier.scopusqualityN/A
dc.identifier.startpage326en_US
dc.identifier.urihttps://doi.org/10.3109/10731199.2012.658469
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5185
dc.identifier.volume40en_US
dc.identifier.wosWOS:000309471000004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherInforma Healthcareen_US
dc.relation.ispartofArtificial Cells Blood Substitutes And Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLaccaseen_US
dc.subjectimmobilizationen_US
dc.subjectpolyacrylamideen_US
dc.subjectcarragennanen_US
dc.subjectsemi-interpenetrating gelen_US
dc.titleImmobilization of laccase on polyacrylamide and polyacrylamide – κ – carragennan-based semiinterpenetrating polymer networksen_US
dc.typeArticle

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