A simple approach for the fabrication of 3D microelectrodes for impedimetric sensing
Yükleniyor...
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Iop Publishing Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this paper, we present a very simple method to fabricate three-dimensional (3D) microelectrodes integrated with microfluidic devices. We form the electrodes by etching a microwire placed across a microchannel. For precise control of the electrode spacing, we employ a hydrodynamic focusing microfluidic device and control the width of the etching solution stream. The focused widths of the etchant solution and the etching time determine the gap formed between the electrodes. Using the same microfluidic device, we can fabricate integrated 3D electrodes with different electrode gaps. We have demonstrated the functionality of these electrodes using an impedimetric particle counting setup. Using 3D microelectrodes with a diameter of 25 mu m, we have detected 6 mu m-diameter polystyrene beads in a buffer solution as well as erythrocytes in a PBS solution. We study the effect of electrode spacing on the signal-to-noise ratio of the impedance signal and we demonstrate that the smaller the electrode spacing the higher the signal obtained from a single microparticle. The sample stream is introduced to the system using the same hydrodynamic focusing device, which ensures the alignment of the sample in between the electrodes. Utilising a 3D hydrodynamic focusing approach, we force all the particles to go through the sensing region of the electrodes. This fabrication scheme not only provides a very low-cost and easy method for rapid prototyping, but which can also be used for applications requiring 3D electric field focused through a narrow section of the microchannel.
Açıklama
GULER, MUSTAFA TAHSIN/0000-0002-0478-3183; Elbuken, Caglar/0000-0001-8359-6871
Anahtar Kelimeler
microfluidic electrical sensing, 3D microelectrodes, flow-focusing, particle counting, microfabrication
Kaynak
Journal Of Micromechanics And Microengineering
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
25
Sayı
9
Künye
Guler, Mustafa & Bilican, Ismail & Agan, Sedat & Elbuken, Caglar. (2015). A simple approach for the fabrication of 3D microelectrodes for impedimetric sensing. Journal of Micromechanics and Microengineering. 25(9), 095019.
