Colloidal stability and biological activity evaluation of microbial exopolysaccharide levan-capped gold nanoparticles

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dc.contributor.authorAkturk, Omer
dc.date.accessioned2021-01-14T18:10:29Z
dc.date.available2021-01-14T18:10:29Z
dc.date.issued2020
dc.departmentKKÜ
dc.description.abstractThe main objectives of this study were to explore the suitability of the exopolysaccharide levan, biosynthesized by Bacillus subtilis, to aid in the formation of gold nanoparticles (AuNPs) and to investigate the colloidal stability and in vitro biological activity of this biopolymer-AuNPs complex. AuNPs (mainly spherical, 8-10 nm-sized, and monodispersed) were successfully synthesized in levan concentrations up to 0.5% w/v (L-AuNP0.5) while exposed to ultraviolet C (UVC) irradiation. The increase of levan quantity decreased the size of AuNPs according to Transmission Electron Microscopy (TEM) images and enhanced the colloidal stability significantly. The presence of L-AuNP0.5 at the highest treatment dose (1000 mu g/mL) exhibited substantial cytotoxicity towards L-929 mouse fibroblasts for all incubation periods. Dose-dependent toxicity was observed for the first day while, after this threshold value, medium (100 mu g/mL) and the lowest (10 mu g/mL) treatment doses were non-cytotoxic during 7 days of incubation, implying dose and time-independent cell viabilities ( > 95%) compared to the negative control (complete cell culture medium). There occurred a special surface interaction with cells and LAuNP0.5, especially when the cells were subjected to deliberate starvation periods to increase L-AuNP0.5 internalization via passive and active endocytosis. Scanning Electron Microscopy (SEM) images showed high accumulation of L-AuNP0.5 around or inside the cell membrane after 7 days. Overall, this attribute (high uptake of L-AuNP0.5) could make them promising candidates for prospective cancer therapeutics or drug delivery systems by enabling the cell internalization of anticancer drugs.en_US
dc.description.sponsorshipScientific Research Projects Unit of Kirikkale UniversityKirikkale University [2017/017]; Scientific and Technological Research Institute of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2209-B, 1139B411700326]en_US
dc.description.sponsorshipThis project was financially supported by the Scientific Research Projects Unit of Kirikkale University (No: 2017/017). This project was initiated and also partly funded by the Scientific and Technological Research Institute of Turkey (TUBITAK), 2209-B Programme (No: 1139B411700326).en_US
dc.identifier.citationBu makale açık erişimli değildir.en_US
dc.identifier.doi10.1016/j.colsurfb.2020.111061
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid32361377
dc.identifier.scopus2-s2.0-85083762985
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2020.111061
dc.identifier.urihttps://hdl.handle.net/20.500.12587/12627
dc.identifier.volume192en_US
dc.identifier.wosWOS:000542568000027
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherELSEVIERen_US
dc.relation.ispartofCOLLOIDS AND SURFACES B-BIOINTERFACES
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLevanen_US
dc.subjectGold nanoparticlesen_US
dc.subjectUVC irradiationen_US
dc.subjectColloidal stabilityen_US
dc.subjectIn vitro bioen_US
dc.subjectlogical activityen_US
dc.titleColloidal stability and biological activity evaluation of microbial exopolysaccharide levan-capped gold nanoparticlesen_US
dc.typeArticle

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