Surface energy components of a dye-ligand immobilized pHEMA membranes: Effects of their molecular attracting forces for non-covalent interactions with IgG and HSA in aqueous media

dc.contributor.authorBayramoglu, G
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:40:16Z
dc.date.available2020-06-25T17:40:16Z
dc.date.issued2005
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn the present paper, we report the study of the adsorption behaviour of human immunoglobulin G (IgG), human serum albumin (HSA) and polyethylenimine (PEI) onto surfaces of Procion Green HE-4BD (PG) immobilized poly(hydroxyethylmethacrylate) (pHEMA) membranes. The adsorption behaviour of the IgG and HSA onto surfaces of the PG-PEI complexed membrane was also studied. Surface wettability and hydrophilicity of all the membranes were investigated by static contact angle measurements. The measurements of the contact angle to various test liquids, i.e., water, glycerol, formamide, diiodomethane (DIM) and ethylene glycol on the investigated membranes were made by sessile drop method. In accordance to the Young equation, the smaller the surface tension of the test liquid, the smaller becomes the contact angles measured on all the investigated membranes surfaces. The highest contact angles were obtained with water, whereas ethylene glycol gave the lowest contact angles for all the tested membranes. Component and parameters of the surface free energy of all the investigated membranes were calculated from measured contact angle values using two methods (the geometric mean by Fowkes and acid-base by van Oss). HSA adsorption was enhanced after complexation of PEI with the immobilized dye-ligand. The adsorption of proteins and PEI significantly changed both the contact angles and component of surface free energies of the investigated membranes. (c) 2005 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.ijbiomac.2005.12.005
dc.identifier.endpage256en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.issue5en_US
dc.identifier.pmid16405992
dc.identifier.scopus2-s2.0-31344477113
dc.identifier.scopusqualityQ1
dc.identifier.startpage249en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2005.12.005
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3366
dc.identifier.volume37en_US
dc.identifier.wosWOS:000235269500005
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal Of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectdye-ligandsen_US
dc.subjectaffinity membranesen_US
dc.subjectHSAen_US
dc.subjectIgGen_US
dc.subjectPEIen_US
dc.subjectadsorptionen_US
dc.subjectcontact angleen_US
dc.subjectsurface energyen_US
dc.titleSurface energy components of a dye-ligand immobilized pHEMA membranes: Effects of their molecular attracting forces for non-covalent interactions with IgG and HSA in aqueous mediaen_US
dc.typeArticle

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