The effect of sodium hyaluronate-chondroitin sulfate combined solution on cartilage formation in osteochondral defects of the rabbit knee: an experimental study

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dc.contributor.authorTosun, Haci Bayram
dc.contributor.authorGurger, Murat
dc.contributor.authorGumustas, Seyit Ali
dc.contributor.authorUludag, Abuzer
dc.contributor.authorUcer, Ozlem
dc.contributor.authorSerbest, Sancar
dc.contributor.authorCelik, Suat
dc.date.accessioned2020-06-25T18:23:38Z
dc.date.available2020-06-25T18:23:38Z
dc.date.issued2017
dc.departmentKırıkkale Üniversitesi
dc.descriptionGurger, Murat/0000-0002-7510-7203
dc.description.abstractObjective: In focal cartilage lesions, multipotent mesenchymal stem cells in bone marrow are aimed to be moved into the defect area using subchondral drilling or microfracture method. However, repaired tissue insufficiently fills the defect area or cannot meet natural hyaline tissue functions, due to fibrous structure. We investigated the effect of a combined solution of sodium hyaluronate + chondroitin sulfate (HA+CS) administered intra-articularly after subchondral drilling on newly formed cartilage in rabbits with focal osteochondral defects. Materials and methods: A total of 32 New Zealand White mature rabbits, whose weights ranged from 2.5 to 3 kg, were randomly divided into four groups. Full-thickness osteochondral defect was formed in the left-knee medial femur condyles of all rabbits. Subchondral drilling was then performed. The following treatment protocol was administered intra-articularly on knee joints on days 7, 14, and 21 after surgery: group 1, 0.3 mL combined solution of HA+CS (20 mg CS combined with 16 mg HA/mL); group 2, 0.3 mL HA (16 mg/mL); group 3, 0.3 mL CS (20 mg/mL); and group 4 (control group), 0.3 mL saline solution. In the sixth week, all animals were killed and then evaluated histopathologically and biochemically. Results: There was significant articular cartilage formation in the HA+CS group compared to the HA, CS, and control groups. Hyaline cartilage formation was observed only in the HA+CS group. Cartilage-surface continuity and smoothness were significantly higher in the HA+CS and HA groups compared to the other groups. Normal cartilage mineralization was found to be significantly higher in the HA+CS group compared to the other groups. Increased levels of VEGFA and IL-1 beta in synovial fluid were observed in the HA+CS group. Conclusion: After subchondral drilling, intra-articular HA-CS combination therapy is a good choice to promote better quality new cartilage-tissue formation in the treatment of focal osteochondral defects.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipWe thank Professor Dr Bilal Ustundag from Firat University Clinical Biochemistry Department, who provided assistance in biochemical analysis and evaluation in this study. This study received financial support from the Scientific and Technological Research Council of Turkey (TUBITAK).en_US
dc.identifier.citationTosun HB, Gürger M, Gümüştaş SA, Uludag A, Üçer Ö, Serbest S, Çelik S. The effect of sodium hyaluronate–chondroitin sulfate combined solution on cartilage formation in osteochondral defects of the rabbit knee: an experimental study. Ther Clin Risk Manag. 2017;13:523-532.en_US
dc.identifier.doi10.2147/TCRM.S133635
dc.identifier.issn1178-203X
dc.identifier.pmid28458555
dc.identifier.scopus2-s2.0-85019672398
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.2147/TCRM.S133635
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7154
dc.identifier.volume13en_US
dc.identifier.wosWOS:000399803700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherDove Medical Press Ltden_US
dc.relation.ispartofTherapeutics And Clinical Risk Management
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcombined therapyen_US
dc.subjectchondroitin sulfateen_US
dc.subjecthyaluronateen_US
dc.subjectosteochondral defecten_US
dc.subjectsubchondral drillingen_US
dc.titleThe effect of sodium hyaluronate-chondroitin sulfate combined solution on cartilage formation in osteochondral defects of the rabbit knee: an experimental studyen_US
dc.typeArticle

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