Preparation of nanofibrous polymer grafted magnetic poly(GMA-MMA)-g-MAA beads for immobilization of trypsin via adsorption

Basit Öğe Kaydı
dc.contributor.authorBayramoglu, Guelay
dc.contributor.authorYilmaz, Meltem
dc.contributor.authorSenel, Ayseguel Uelkue
dc.contributor.authorArica, M. Yakup
dc.date.accessioned2020-06-25T17:44:33Z
dc.date.available2020-06-25T17:44:33Z
dc.date.issued2008
dc.departmentKırıkkale Üniversitesi
dc.description.abstractPoly (glycidylmethacrylate-methylmethacrylate), poly(GMA-MMA) beads were prepared via suspension polymerization in the presence of ferric ions. The epoxy groups of the poly(GMA-MMA) beads were converted into amino groups during magnetization reaction, and then were grafted with methacrylic acid (MAA) via graft copolymerization. The magnetic beads were characterized by surface area measurement, swelling test, scanning electron microscope (SEM), electron spin resonance (ESR) and Mossbauer spectroscopy. The enzyme "trypsin" was immobilized on the magnetic beads via adsorption. The maximum adsorption was obtained at pH 7.0. At 2.0 mg/mL initial trypsin concentration, the maximum immobilization capacity was 123.2 mg trypsin/g beads and retained about 84.2% of its initial activity. The immobilized trypsin could not be desorbed by enzyme reaction solution in the pH range of 5.0-9.0, and could be desorbed by 1.0 M formic acid solution containing 1 M NaCl. (C) 2007 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.bej.2007.12.013
dc.identifier.endpage274en_US
dc.identifier.issn1369-703X
dc.identifier.issn1873-295X
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-43849084570
dc.identifier.scopusqualityQ2
dc.identifier.startpage262en_US
dc.identifier.urihttps://doi.org/10.1016/j.bej.2007.12.013
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4140
dc.identifier.volume40en_US
dc.identifier.wosWOS:000257228100010
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmagnetic beadsen_US
dc.subjectadsorptionen_US
dc.subjectenzyme technologyen_US
dc.subjectproteaseen_US
dc.subjectproteinen_US
dc.subjectpeptide mapsen_US
dc.titlePreparation of nanofibrous polymer grafted magnetic poly(GMA-MMA)-g-MAA beads for immobilization of trypsin via adsorptionen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Preparation of nanofibrous polymer grafted magnetic poly(GMA-MMA)-g-MAA beads for immobilization of trypsin via adsorption.pdf
Boyut:
1.36 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu